G B Pierson

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We have used cryo-electron microscopy of vitrified specimens to study microtubules assembled both from three cycle purified tubulin (3 x-tubulin) and in cell free extracts of Xenopus eggs. In vitro assembled 3x-tubulin samples have a majority of microtubules with 14 protofilaments whereas in cell extracts most microtubules have 13 protofilaments.(More)
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