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An epidemiological survey was made of 5329 samples from 10 poultry operations to determine the relationship between total poultry farm environment and incidences of Salmonella contamination of broiler flocks. Samples were analyzed from walls, drinkers, feeders, litter, insects, water, chicks, broilers, and feed to determine the effect of common sanitary(More)
1. Four groups of hens, each of a different line, were inoculated at peak of lay, per os in the crop with 1 ml of a suspension containing 10(9) cfu/ml Salmonella enteritidis PT4 (SE). The kinetics of SE contamination in the environment, egg shell and yolk were studied during the first 28 d post inoculation. On the day of slaughter, intestines, caeca,(More)
Two enzyme-linked immunosorbent assays (ELISA) developed in the authors' laboratory for turkey rhinotracheitis serological testing, a commercial ELISA kit, and two virus-neutralization (VN) assays were compared with respect to the efficiency of these assays for serological monitoring in specific-pathogen-free (SPF) turkeys inoculated with four pathogenic(More)
This report describes an ELISA blocking test using a peroxidase-labelled monoclonal antibody which binds to the HN protein of Newcastle disease (NDV). This test allows specific detection of type 1 avian paramyxovirus (PMV1) antibodies but does not detect other avian paramyxovirus (PMV2-9) antibodies recognized by the usual serological NDV tests (HI,(More)
Avoparcin (10 mg/kg feed), bacitracin (50 mg/kg), flavomycin (5 mg/kg) and virginiamycin (20 mg/kg) were tested for their synergy or antagonism on the protective effect of an adult caecal flora administered to 1-day-old chicks. The chicks were challenged experimentally per os with 10(4) to 10(5)Salmonella typhimurium (a rifampicin-resistant strain) when(More)
On the basis of the aligned 16S rRNA sequences of Mollicutes, a pair of primers was chosen for the detection of Mycoplasma gallisepticum. When used in the polymerase chain reaction (PCR), the primers detected a specific amplification of all Mg strains tested, yielding an expected 330 bp product. Amplification was not detected when other Mollicutes or E.(More)
This paper describes the isolation and identification of a duck plague virus (DP) and a paramyxovirus (PMV6), from the livers and intestines collected in 4-month old mule ducks, under fattening, exhibiting 75% mortality and necrotic-haemorrhagic gross lesions. These viruses were isolated in specific pathogen free (SPF) muscovy duck eggs and SPF chicken eggs(More)
Virological 1 examination of a severe infectious bronchitis (IB)-like field case in laying hens, led to the isolation of a coronavirus antigenically different from Massachusetts, Connecticut and four Dutch IB variant strains. The virulence of the isolate for the fowl, and its dual tropism for the respiratory and genital tracts were demonstrated. In(More)