G. Akusjarvi

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  • G. Akusjarvi
  • Frontiers in bioscience : a journal and virtual…
  • 2008
Adenovirus makes extensive use of alternative RNA splicing to produce a complex set of spliced mRNAs during replication. The accumulation of viral mRNAs is subjected to a temporal regulation, a mechanism that ensures that proteins that are needed at certain stages of the virus life cycle are produced in a timely fashion. The complex interactions between the(More)
We show that transformation of rat CREF fibroblasts by adenovirus-2 early regions 1A and 1B (E1) is stimulated by expression of the viral early region 4 (E4) products. Cotransfection of CREF cells with E1 and E4 did not affect the number of E1-transformed foci, but resulted in the formation of larger and more dense foci compared to E1 transfection alone.(More)
Vepatocellular carcinoma (tlCC) is anm'g the ten most frequent cancers worldwide, with a high incidence in south-esst Asia and parts of Africa, and the major risks factors are hepatitis B and C viral ir~ection and exposure to aflatoxins. Vaccination against HBV is effective in the prevention of ~ infection and various vaccination projects are trader way in(More)
SR proteins are essential splicing factors required for constitutive splicing and function as key regulators of alternative RNA splicing. We have shown that SR proteins purified from late adenovirus-infected cells (SR-Ad) are functionally inactivated as splicing enhancer or splicing repressor proteins by a virus-induced partial de-phosphorylation. Here, we(More)
We report the sequence of a 1164 nucleotide long DNA segment, located between map positions 59.5 and 62.8 on the adenovirus type 2 genome. The sequence comprises the 701 nucleotides long 3' non-coding region of the hexon mRNA as well as several important processing signals. The sequence revealed unexpectedly that the 3' non-coding region of the hexon mRNA(More)
We have developed a sensitive transient expression assay in 293 cells to study the effect of VA RNAs on the translation of adenovirus mRNAs. Monolayers of 293 cells were transfected with mixtures of recombinant plasmids encoding adenovirus-specific transcription units and plasmids encoding VA RNAs. Transfected cells were labeled with [35S]methionine for ca.(More)
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