G. A. Velikanov

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This paper presents a review of modern data on the functional designation of membrane contact sites (MCSs) of endoplasmic reticulum. Problems of traffic of lipids and calcium in are discussed. It is to be emphasized that the function of MCSs is not yet clear, while the mechanism providing contact of two membranes (the problem of “anchors”) remains poorly(More)
Effect of exogenous ABA and an inhibitor of energy metabolism NaN3 on water permeability of the desmotubules and tonoplast as the structural elements of vacuolar symplast ensuring water permeability of this transport system was investigated. The methodological approach based on the use of NMR with magnetic field pulse gradient is described in detail. It was(More)
Indirect immunofluorescent microscopy and a tonoplast-specific marker enzyme were used to demonstrate the occurrence of pyrophosphatase within the plasmodesmata in the elongation zone of maize root segments. The pulsed field gradient NMR method (PFG NMR) was applied to study restricted self-diffusion of water molecules in the root segments under normal(More)
New concepts of structural-functional organization of the transport system in higher plants were evolved at the current stage of investigations. In addition to the classical (cytoplasmic) symplast, another supra-cellular continuum was supposed to exist in the plant tissue, which interconnects vacuoles of neighboring cells through desmotubules and represents(More)
Radial self-diffusion of water in the absorbing zone of the roots of winter wheat (Triticum aestivumL.) seedlings was studied by the pulse-gradient-spin-echo NMR method. At the fixed time of diffusion observation, the diffusion decay of proton spin-echo was nonexponential; however, it could be reliably separated into three exponential components differing(More)
Close contacts of the endoplasmic reticulum membrane and plasmalemma have been visualized inside plant cells by means of electron microscopy. The qualitative similarity of these contacts to high-permeable intercellular contacts in animals has been shown. New data confirming the hypothesis of the identity of stromules, i.e., dynamic tubular protuberances of(More)
The problem of the existence of a continuous transcellular association of internal spaces of endoplasmic reticulum (ER) and central vacuoles of next cells into a single system (vacuolar symplast) containing within the plastids and mitochondria in plant tissues is discussed. Based on the original experimental data obtained by NMR spectroscopy and electron(More)
An NMR method with a pulsed magnetic field gradient was applied to study changes in water permeability of the vacuolar symplast in maize (Zea mays L.) seedling roots treated with various inhibitors of cell metabolism. The results were qualitatively analogous to literature data on conductivity changes of intercellular gap junctions in animal cells exposed to(More)