Günter Wallner

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A combination of fluorescent rRNA-targeted oligonucleotide probes ("phylogenetic stains") and flow cytometry was used for a high resolution automated analysis of mixed microbial populations. Fixed cells of bacteria and yeasts were hybridized in suspension with fluorescein- or tetramethylrhodamine-labeled oligonucleotide probes complementary to(More)
In situ identification of whole fixed bacterial cells by hybridization with fluorescently labeled, rRNA-targeted oligonucleotide probes is often limited by low signal intensities. In addition to an impermeability of the cell periphery and a low cellular rRNA content, the three-dimensional structure of the ribosome may hinder the access of oligonucleotides(More)
Not only classical cultivation-based methods but also the new molecular approaches may result in incomplete and selective information on the natural diversity of microbial communities. Flow sorting of microorganisms from environmental samples allows the deliberate selection of cell populations of interest from highly diverse systems for molecular analysis.(More)
In this paper we present "Homie" an artificial companion for elderly people. Our approach emphasizes amusement and benefit - amusement in form of entertainment and benefit in terms of medical care. The key to awake elderly people's emotional engagement in an artificial companion is its emotional behavior. Therefore, we propose a companion that does not look(More)
As video games are becoming more and more complex and are reaching a broader audience, there is an increasing interest in procedures to analyze player behavior and the impact of design decisions. Game companies traditionally relied on user-testing methods, like playtesting, surveys or videotaping, to obtain player feedback. However, these qualitative(More)
With increasing popularity of ontologies in various communities, visualizations of their content and structure became more and more important. In the past few years a number of visualization approaches were developed with the focus either on the representation of the relationships between classes or on the hierarchical structure and instances. However for(More)
Samples from a wastewater treatment plant were hybridized with fluorescein-labeled oligonucleotide probes specific for members of the domains Bacteria and Eucarya; the alpha, beta, and gamma subclasses of the class Proteobacteria; or the genus Acinetobacter. Subsequently, they were counterstained with the DNA-specific dye Hoechst 33342 and analyzed by flow(More)
Oligonucleotides were end-labelled with digoxigenin (DIG), chemically at the 5'-end or enzymically at the 3'-end. Following specific in situ hybridization of these probes to intracellular rRNA molecules, the hybrids were detected with anti-DIG Fab fragments labelled with fluorescent dyes. The antibody fragments penetrated through the bacterial cell(More)
Contemporary video games are highly complex systems with many interacting variables. To make sure that a game provides a satisfying experience, a meaningful analysis of gameplay data is crucial, particularly because the quality of a game directly relates to the experience a user gains from playing it. Automatic instrumentation techniques are increasingly(More)