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The goal of the present study was to elucidate the ionic mechanisms by which cholinergic stimulation induces cell shrinkage in eccrine clear cells. Dissociated Rhesus monkey eccrine sweat clear cells were prepared by collagenase digestion of freshly isolated secretory coils and immobilized on a glass slide in a perfusion chamber at 30°C. The cell was(More)
Methacholine (MCh)-induced changes in intracellular concentrations of Na, K, and Cl ([Na]i, [K]i, and [Cl]i, respectively) and in cellular dry mass (a measure of cell shrinkage) were examined in isolated monkey eccrine sweat secretory coils by electron probe X-ray microanalysis using the peripheral standard method. To further confirm the occurrence of cell(More)
Tissue cyclic 3′5′-AMP (cAMP) concentrations were measured after stimulation of isolated monkey palm eccrine sweat glands with various stimulants of sweat secretion. The cellular cAMP levels increased curvilinearly with time of incubation to reach a steady state after 5 min of incubation with isoproterenol. Theophylline more than doubled the(More)
Using the whole-cell voltage clamp (to determine the membrane current) and current clamp (to determine membrane potential) methods in conjunction with the nystatin-perforation technique, we studied the effect of methacholine (MCh) and other secretagogues on whole cell K and Cl currents in dissociated rhesus palm eccrine sweat clear cells. Application of MCh(More)
Cystic fibrosis transport regulator is a cAMP-dependent chloride channel protein. Normal (non cystic fibrosis) human epidermis stained positive for cystic fibrosis transport regulator as densely as did the eccrine sweat gland when three monoclonal antibodies for R (regulatory) and C (C-terminus) domains of cystic fibrosis transport regulator were used. All(More)
Durch Epinephrine stimulierte menschliche Schweissdrüsen produzierten ein Sekret, welches 35–45 mÄq/l Natriumionen, 10–18 mÄq/l Kaliumionen und 15–19 mÄq/l Lactat enthielt. Adrenergisch stimulierter Schweiss hat demnach eine ähnliche Zusammensetzung wie cholinergisch stimulierter Schweiss. Mit dem Elektronenmikroskop liess sich nach der Stimulation durch(More)
Several mathematical cervical models of the 50th percentile male have been developed and used for impact biomechanics research. However, for the 50th percentile female no similar modelling efforts have been made, despite females being subject to a higher risk of soft tissue neck injuries. This is a limitation for the development of automotive protective(More)
OBJECTIVE The aim of this study was to investigate the whole spine alignment in automotive seated postures for both genders and the effects of the spinal alignment patterns on cervical vertebral motion in rear impact using a human finite element (FE) model. METHODS Image data for 8 female and 7 male subjects in a seated posture acquired by an upright open(More)
A liver Finite Element (FE) model with hyper‐viscoelastic properties was developed. Hyper‐elastic and rate‐dependent characteristics were modeled with an Ogden rubber material model. Such characteristics were validated against an original series of porcine exsanguinated livers under quasi‐static and dynamic compression experiments. The applicability of the(More)
OBJECTIVES The ultimate goal of this research is to reduce thoracic injuries due to traffic crashes, especially in the elderly. The specific objective is to develop and validate a full-body finite element model under 2 distinct settings that account for factors relevant for thoracic fragility of elderly: one setting representative of an average size male(More)
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