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High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases
Methods for using simple ssDNA oligonucleotides in tandem with ZFNs to efficiently produce human cell lines with three distinct genetic outcomes: targeted point mutation, targeted genomic deletion of up to 100 kb and targeted insertion of small genetic elements concomitant with large genomic deletions.
Molecular organization of a gene in barley which encodes a protein similar to aspartic protease and its specific expression in nucellar cells during degeneration
The cloned and characterized a barley gene which encodes an aspartic protease-like protein and is specifically expressed in nucellar cells during degeneration, and this protein is named ‘nucellin’, which may be involved withnucellar cell death.
A collection of sequenced and mapped Ds transposon insertion sites in Arabidopsis thaliana
A collection of Dissociation(Ds) transposon lines with insertions on all 5 Arabidopsischromosomes is generated, which constitutes the largest collection of sequence-mapped Ds insertion sites unbiased by selection against the donor site.
Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting
- Fuqiang Chen, Xiao Ding, Yongmei Feng, Timothy Seebeck, Yanfang Jiang, G. Davis
- BiologyNature communications
- 7 April 2017
This work shows that the type II-B Fn Cas9 from Francisella novicida possesses novel properties, but its nuclease function is frequently inhibited at many genomic loci in living human cells, and develops a proximal CRISPR (termed proxy-CRISPR) targeting method that restores FnCas9 nucleasing activity in a target-specific manner.
Improving CRISPR-Cas9 Genome Editing Efficiency by Fusion with Chromatin-Modulating Peptides.
- Xiao Ding, Timothy Seebeck, Yongmei Feng, Yanfang Jiang, G. Davis, Fuqiang Chen
- BiologyThe CRISPR journal
- 21 February 2019
Modification of the widely used Streptococcus pyogenes Cas9 by fusion with chromatin-modulating peptides (CMPs), derived from high mobility group proteins HMGN1 and HMGB1, histone H1, and chromatin remodeling complexes, improves its activity by up to several fold, particularly on refractory target sites.
Gene editing using ssODNs with engineered endonucleases.
- Fuqiang Chen, Shondra M. Pruett-Miller, G. Davis
- Biology, EngineeringMethods in molecular biology
The use of ssODNs is described for making defined genome modifications in combination with engineered endonucleases to obviate the more laborious and time-consuming plasmid vector construction process.
Screening key genes and signaling pathways in colorectal cancer by integrated bioinformatics analysis
- Chang-Yung Yu, Fuqiang Chen, Jianjun Jiang, Hong Zhang, Meijuan Zhou
- BiologyMolecular medicine reports
- 4 June 2019
Insight is provided into the molecular mechanism of CRC that may be useful in further investigations and survival analysis revealed that TOP2A, MAD2L1, CDC6 and CHEK1 may serve as prognostic biomarkers in CRC.
Effect of exogenous plant growth regulators on in vitro seed growth, embryo development, and haploid production in a cross between H. vulgare (L.) and H. bulbosum (L.)
2,4-D alone or kinetin +2, 4-D are recommended for the purpose of barley haploid production in floret culture using the bulbosum method.
Cardiac Glycoside Ouabain Exerts Anticancer Activity via Downregulation of STAT3
- Jie-Zhen Du, Lijun Jiang, Fuqiang Chen, Huantao Hu, Meijuan Zhou
- Biology, ChemistryFrontiers in Oncology
- 30 June 2021
A novel molecular insight of anticancer activities of ouabain in human cancer cells is provided, which could raise the hope of using cardiac glycosides for cancer therapeutics more rational.
GenElute TM Five-Minute Plasmid Miniprep Kit : The Fastest Method for Plasmid Preparation
- Fuqiang Chen
Mini preparations of plasmid DNA are sufficient in most basic experiments, but each preparation can take about 20-30 minutes and include a lot of hands-on steps.