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Transient transformation is simpler, more efficient and economical in analyzing protein subcellular localization than stable transformation. Fluorescent fusion proteins were often used in transient transformation to follow the in vivo behavior of proteins. Onion epidermis, which has large, living and transparent cells in a monolayer, is suitable to(More)
A full-length cDNA (SsFAD3) for an omega-3 fatty acid desaturase (omega-3 FAD) was cloned from Sapium sebiferum (L.) Roxb. using rapid amplification of cDNA ends and reverse transcription polymerase chain reaction methods. SsFAD3 contained a 1119-bp open reading frame encoding a 372-amino acid polypeptide. The genomic sequence region of the SsFAD3 ORF was(More)
An optimized regeneration and Agrobacterium-mediated transformation protocol based on whole cotyledonary node explants was developed in soybean (Glycine max) cultivar Zhong Huang 13. Adding 6-benzylaminopurine (BAP) in a germinating medium could significantly increase regeneration efficiency; the optimal BAP concentration for shoot formation was 0.5 mg/L.(More)
Radiotherapy is an effective treatment for some esophageal cancers, but the molecular mechanisms of radiosensitivity remain unknown. Ubiquitin-like with PHD and ring finger domains 1 (UHRF1) is a novel nuclear protein which is overexpressed in various cancers but not yet examined in esophageal squamous cell carcinoma (ESCC). The correlation between UHRF1(More)
To investigate the expression of the Toll-like receptor (TLR) 4 in the brain after experimental subarachnoid haemorrhage (SAH) in rabbits. A total of 52 rabbits were randomly divided into four groups: control group; day 3, day 5, and day 7 groups. Day 3, day 5, and day 7 groups were all SAH groups in which the rabbits were killed on day 3, 5, and 7,(More)
A variable index metamaterial is demonstrated by embedding nematic liquid crystal inside fishnet layers' void at microwave frequencies. With an external electric field, the left handed passband can be reversibly shifted from 9.14 to 8.80 GHz, whereas the upper right handed passband is nearly unchanged. It is shown that during LC molecular reorientation,(More)
Francisella tularensis is a potential biowarfare/bioterrorism agent and zoonotic pathogen that causes tularemia; thus, surveillance of F. tularensis and first-level emergency response using point-of-care testing (POCT) are essential. The UPT-LF POCT assay was established to quantitatively detect F. tularensis within 15 min, and the sensitivity of the assay(More)
INTRODUCTION The use of conservative surgery combined with whole-breast irradiation (WBI) has been established as a valid alternative to mastectomy for the management of early-stage breast cancer. The aim of this study was to compare dosimetric parameters of the planning target volume(PTV) and organs at risk (OARs) between conventional radiation therapy(More)
Graphene has drawn considerable attention due to its intriguing properties in photonics and optoelectronics. However, its interaction with light is normally rather weak. Meta-surfaces, artificial structures with single planar function-layers, have demonstrated exotic performances in boosting light-matter interactions, e.g., for absorption enhancement.(More)
A new protocol was established for the regeneration of Solanum nigrum by frog egg-like bodies (FELBs), which are novel somatic embryogenesis (SE) structures induced from the root, stem, and leaf explants. The root, stem, and leaf explants (93.33%, 85.10%, and 100.00%, respectively) were induced to form special embryonic calli on Murashige and Skoog (MS)(More)