Fu-liang Cao

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Agroforestry practice is believed to be an effective means of maintaining and improving soil fertility, and is widely used by farmers around the world. To gain better understanding of the effects of agroforestry practice on soil fertility, the organic carbon content, total nitrogen content, microbial biomass, basal respiration, and activity of soil enzymes(More)
The cinnamyl alcohol dehydrogenase (CAD) is a key enzyme in lignin biosynthesis as it catalyzes the final step in the synthesis of monolignols. A cDNA sequence encoding the CAD gene was isolated from the leaves of Ginkgo biloba L, designated as GbCAD1. The full-length cDNA of GbCAD1 was 1,494 bp containing a 1,074 bp open reading frame encoding a(More)
Intercropping Ginkgo and crop species in southern China is receiving increasing attention because it offers potential advantages for resource utilization, higher economic income to farmers and increased sustainability in crop production, We carried out a 2-year field intercropping system composed of Ginkgo with wheat, broad bean, and rapeseed, respectively,(More)
Ginkgo suspension cells were used to investigate the mechanism that governs the shift between primary and secondary metabolism under NaCl elicitation. The production of three flavonol glycosides, chlorophyll fluorescence, ion content, the antioxidant system, and the cellular ultrastructure in the presence of NaCl doses from 5 to 175 mM were examined. At low(More)
Heat shock proteins (HSPs) play various stress-protective roles in plants. In this study, three HSP genes were isolated from a suppression subtractive hybridization (SSH) cDNA library of Ginkgo biloba leaves treated with cold stress. Based on the molecular weight, the three genes were designated GbHSP16.8, GbHSP17 and GbHSP70. The full length of the three(More)
Ginkgo (Ginkgo biloba L.) exhibits high level of resistance to various abiotic stresses, yet has undergone only minor morphological changes through millions of years. The conversion of membrane lipid components may play an important role in the temperature acclimation of plants. Stearoyl-ACP desaturase, microsomal ω-6 (Δ12) desaturase, and plastidial ω-6(More)
Response of growth and secondary metabolites to light intensity are useful measurements to determine suitable silviculture treatments for the cultivation of medicinal plants. Here, we analyzed the growth, flavonols (total flavonol, quercetin, kaempferol, and isorhamnetin) content, flavonols yield per plant, and expression of flavonoid biosynthesis-related(More)
Rare earth elements, a type of abiotic elicitor, were used to investigate the shift in the mechanism of flavonoid accumulation from primary metabolites in Ginkgo suspension cell culture. The changes in flavonoids, mineral ions, photosynthetic pigments, chlorophyll fluorescence and antioxidant enzymes with varying Ce3+ (Ce(NO3)3) doses (0.01–5.0 mM) were(More)
Ginkgo biloba is a dioecious species native to China with medicinally and phylogenetically important characteristics; however, genomic resources for this species are limited. In this study, we performed the first transcriptome sequencing for Ginkgo kernels at five time points using Illumina paired-end sequencing. Approximately 25.08-Gb clean reads were(More)
The flavonoids content and its composition in Ginkgo (Ginkgo biloba L.) leaves are affected by environmental factors such as temperature and soil moisture. Here we performed experiments in phytotron using 2-year-old Ginkgo seedlings to explore the effects of temperature and soil moisture on flavonoids content, enzymes related to flavonoids biosynthesis such(More)