Friedhelm Kühn

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Like true DNA replicases, herpes simplex virus type 1 DNA polymerase is equipped with a proofreading 3'-5'-exonuclease. In order to assess the functional significance of conserved residues in the putative exonuclease domain, we introduced point mutations as well as deletions within and near the conserved motifs' exonuclease (Exo) I, II, and III of the DNA(More)
An epitope (HPOL) derived from the so-called thumb region of the herpes simplex virus type 1 DNA polymerase in combination with a monoclonal antibody (MAb 1051c) was tested for protein tagging. Using a conventional expression vector, a DNA cassette encoding the HPOL epitope was fused to the C-terminus of the dihydrofolate reductase (DHFR) gene such that the(More)
Unternehmen haben immense Summen in die Entwicklung ihrer Anwendungssysteme investiert und hängen vom zuverlässigen und wirtschaft lichen Betrieb dieser Anwendungen erschließt sich die Mehrzahl der liegenden Quellcode enthaltenen Fakten arbeitern der IT-Abteilungen nur schwer von XCMR (Extensible Code Mining Repository), einem generischen(More)
Herpes simplex virus DNA polymerase (HSV pol) holoenzyme consists of a large catalytic (UL30 gene product) and a small auxiliary subunit (UL42 gene product). The DNA binding of HSV pol, its cofactor, and the assembled holoenzyme complex was studied by bandshift analysis using purified proteins expressed via recombinant baculovirus. The functional activity(More)
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