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In the mammalian retina, light signals generated in photoreceptors are passed to bipolar and horizontal cells via synaptic contacts. In various pathological conditions, these second-order neurons extend neurites into the outer nuclear layer (ONL). However, the molecular events associated with this neurite outgrowth are not known. Here, we characterized the(More)
RT6 is an unusual cell membrane protein that is expressed exclusively by postthymic T cells. The inherent defect in its expression has been correlated to lymphopenia and genetically determined susceptibility for insulin-dependent diabetes mellitus in the rat. We report here the primary structure of the RT6.2 alloantigen as deduced from the cDNA sequence.(More)
The rat T-cell alloantigenic system RT6 comprises two known alleles, RT6 and RT6. The respective protein products, designated RT6.1 and RT6.2, are expressed on mature peripheral T-cells of the rat, but not on thymocytes or recent thymic migrants (1—3). Diabetes-prone BB rats are deficient in RT6 expression, and this defect has been correlated with the(More)
A single-step immunoaffinity purification procedure for the rat T cell marker RT6.2 is described which permits the isolation of microgram quantities of protein from the RT6.2+ T-T hybridoma EpD3. The amino terminus was sequenced directly from a polyvinylidene (PVDF) membrane blot prepared after SDS-PAGE. Further internal sequence data were obtained from(More)
Cell death in neurodegenerative diseases is often thought to be governed by apoptosis; however, an increasing body of evidence suggests the involvement of alternative cell death mechanisms in neuronal degeneration. We studied retinal neurodegeneration using 10 different animal models, covering all major groups of hereditary human blindness (rd1, rd2, rd10,(More)
The mechanism by which the rat T cell alloantigen, RT-6.2, is attached to the membrane was investigated. Treatment of rat lymph node and T-hybridoma cells with phosphatidylinositol-specific phospholipase C (PI-PLC) caused a substantial reduction in the amount of RT-6.2 on the cell surface. No significant release of a rat T helper marker (visualized by the(More)
Utilizing allotype-specific antibodies to immunoprecipitate RT6.2 from DA.6B rat lymphocyte lysates, we have shown this antigen recently to be composed of two related, non-glycosylated polypeptides with apparent molecular weights (MW) of 24,000 and 26,000 (reducing conditions), which evidently are anchored in the cell membrane by covalent linkage to(More)
The phosphatidylinositol-linked T-cell surface protein RT6 occurs in two alloantigenic forms, RT6.1 and RT6.2. A lambda gt11 cDNA expression library was constructed from the RT6.2+ T-T hybridoma cell line EpD3 and screened with RT6-specific antisera. One of nine independent positive clones obtained in an initial screening of 500,000 recombinant phages was(More)