František Adamec

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Photosynthetic organisms exposed to a dynamic light environment exhibit complex transients of photosynthetic activities that are strongly dependent on the temporal pattern of the incident irradiance. In a harmonically modulated light of intensity I approximately const.+sin(omegat), chlorophyll fluorescence response consists of a steady-state component, a(More)
Results of low temperature fluorescence and spectral hole burning experiments with whole cells and isolated chlorosomes of the green sulfur bacterium Chlorobium limicola containing BChl c are reported. At least two spectral forms of BChl c (short-wavelength and long-wavelength absorbing BChl c) were identified in the second derivative fluorescence spectra.(More)
Fluorescence resonance energy transfer (FRET) under in vivo conditions is a well-established technique for the evaluation of populations of protein bound/unbound nucleic acid (NA) molecules or NA hybridization kinetics. However, in vivo FRET has not been applied to in vivo quantitative conformational analysis of NA thus far. Here we explored parameters(More)
Net photosynthetic rate (PN), productivity and the first phases of the fluorescence induction curve were investigated in leaves of two potato cultivars exposed to water stress. Water stress applied to potato plants at the beginning of their development (planting-bud formation) increased productivity but decreased PN and variable fluorescence (Fv) of leaves.(More)
For more than a century, mechanisms of photosynthesis in plants and in autotrophic bacteria have been studied using fluorescence emission of chlorophyll and of other light-harvesting pigments. This long tradition as well as uniqueness of photosynthetic processes led to a separate development of fluorescence techniques in plant science and in other fields of(More)
The recent advancements in life sciences are inseparably associated with the ongoing progress of mainstream physical measurement methodologies, biochemistry and physical chemistry, and the novel nanofabrication techniques. The reviewed book covers essential technological details of a selection of biophysical methods in a rare brilliant way. It is best(More)
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