Frans A.A. Tetteroo

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Plant somatic embryos usually lack desiccation tolerance. They may acquire such a tolerance upon preculture in the presence of abscisic acid (ABA), followed by slow drying, but not fast drying. ABA causes torpedo-shaped somatic embryos to lose their chlorophyll, suspend growth, exhibit low rates of respiration, and maintain elevated sucrose contents. The(More)
Abscisic acid-pretreated carrot (Daucus carota) somatic embryos survive dehydration upon slow drying, but fast drying leads to poor survival of the embryos. To determine whether the acquisition of desiccation tolerance is associated with changes in the physical stability of the cytoplasm, in situ Fourier transform infrared microspectroscopy was used.(More)
Carrot (Daucus carota) somatic embryos that were treated with abscisic acid during their development have been shown to acquire complete desiccation tolerance when slowly dried, but fail to do so when rapidly dried. We studied plasma membrane permeability by a spin probe technique. On rehydration, the plasma membranes of rapidly dried somatic embryos became(More)
Methods are described for the vegetative propagation of Begonia venosa Skan. Young flower buds are capable of producing callus which, contrasting to callus from leaves of adult plants, is very organogenic. For callus induction are required: BA and NAA at a conc. of 0.5 mgl−1, 21 °C and low irradiance. Subculture of organogenic callus is optimal on a medium(More)
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