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The GTP-dependent restriction enzyme McrBC from Escherichia coli forms high-molecular mass complexes with DNA and produces a cleavage pattern with a characteristic 10-base pair repeat.

• Uwe Pieper, Detlef H Groll, +4 authors Alfred Pingoud
• Biochemistry
• 2002

The GTP-dependent restriction enzyme McrBC consists of two polypeptides: one (McrB) that is responsible for GTP binding and hydrolysis as well as DNA binding and another (McrC) that is responsible… (More)

Genetic engineering of Escherichia coli to produce a 1:1 complex of the anabaena sp. PCC 7120 nuclease NucA and its inhibitor NuiA.

• Christian Understanding’Eugene Korn, Gregor Meiss, Frank-Ulrich Gast, Oleg A. Gimadutdinow, Claus Urbanke, Alfred Pingoud
• Gene
• 2000

A series of T7-promoter based bicistronic expression vectors was constructed in order to produce the complex of the Anabaena sp. PCC 7120 DNA/RNA non-specific nuclease NucA and its inhibitor NuiA.… (More)

DNA Computing in Microreactors

• Danny van Noort, Frank-Ulrich Gast, John S. McCaskill
• DNA
• 2001

The goal of this research is to improve the programmability of DNA-based computers. Novel clockable microreactors can be connected in various ways to solve combinatorial optimisation problems, such… (More)

A New Structural Motif in the Left Terminal Domain of Large Viroids Identified by Covariation Analysis

• Frank-Ulrich Gast
• Virus Genes
• 2003

Sequence alignment of non-hammerhead viroids (Pospiviroidae) reveals that they can be divided into large and small viroids. Covariation analysis of these groups provides evidence for a rod-like… (More)

A Macintosh program for the versatile generation of random nucleic acid sequences and their structural analysis

• Frank-Ulrich Gast
• Computer Applications in the Biosciences
• 1994

The program 'MacStAn' for the Apple Macintosh generates random sequences and can analyze their tendency to form secondary structure or translation products as well as their mono-, di- and… (More)

Viroids proper can be distinguished from hammerhead viroids and satellite RNAs through their dinucleotide composition

• Frank-Ulrich Gast, R. L. Spieker
• Archives of Virology
• 1996

G + C-rich viroids proper exhibit a unique dinucleotide composition in that AU and UA are much less frequent than expected. Thus, evaluation of the dinucleotide pattern allows a quick discrimination… (More)

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