Frank N Crespilho

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An intravenous implantable glucose/dioxygen hybrid enzyme-Pt micro-biofuel cell (BFC) was investigated. In this miniaturized BFC, a flexible carbon fiber (FCF) microelectrode modified with neutral red redox mediator and glucose oxidase was used as the bioanode, and an FCF modified with platinum nanoparticles stabilized on PAMAM-G4 dendrimer was used as the(More)
Clinical diagnosis has always been dependent on the efficient immobilization of biomolecules in solid matrices with preserved activity, but significant developments have taken place in recent years with the increasing control of molecular architecture in organized films. Of particular importance is the synergy achieved with distinct materials such as(More)
This paper brings an overview of the use of nanostructured films in several types of biosensors, with emphasis on the advantageous control of molecular architecture which is typical of the layer-by-layer (LbL) and Langmuir-Blodgett films. Following introductory sections on film fabrication and detection methods, we concentrate on the immobilization of(More)
This paper provides an overview of different nanostructured architectures utilised in electrochemical devices and their application in biosensing and bioelectronics. Emphasis is placed on the fabrication of nanostructured films based on a layer-by-layer (LBL) films approach. We discuss the theory and the mechanism of charge transfer in polyelectrolyte(More)
We show a simple strategy to obtain an efficient enzymatic bioelectrochemical device, in which urease was immobilized on electroactive nanostructured membranes (ENMs) made with polyaniline and silver nanoparticles (AgNP) stabilized in polyvinyl alcohol (PAni/PVA-AgNP). Fabrication of the modified electrodes comprised the chemical deposition of polyaniline(More)
The immobilization of enzymes in organized two-dimensional matrices is a key requirement for many biotechnological applications. In this paper, we used the Langmuir-Blodgett (LB) technique to obtain controlled architectures of urease immobilized in solid supports, whose physicochemical properties were investigated in detail. Urease molecules were adsorbed(More)
The concept of constitutional dynamic chemistry (CDC) based on the control of non-covalent interactions in supramolecular structures is promising for having a large impact on nanoscience and nanotechnology if adequate nanoscale manipulation methods are used. In this study, we demonstrate that the layer-by-layer (LbL) technique may be used to produce(More)
This paper presents studies about the molecular interactions and redox processes involved in the formation of palladium nanoparticles associated to glucose oxidase (GOx-PdNPs) in a supramolecular arrangement. The synthesis occurs in two steps, the Pd reduction and the formation of the 80 nm sized supramolecular aggregates containing multiples units of GOx(More)
O direcionamento por campo magnético tem sido aplicado em Bioeletroquímica, principalmente com intuito de transportar enzimas ou mediadores redox imobilizados em partículas de magnetita (Fe3O4) para a superfície de eletrodos, proporcionando o controle comutável das correntes faradaicas provenientes da biocatálise. Neste trabalho, relata-se um avanço no(More)