Frank H. Mukai

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Incubation of histidine requiring auxotrophs of the bacterium Salmonella typhimurium with malonaldehyde, a three-carbon dialdehyde, produced an increased number of revertants in specific strains. Mutagenesis was only observed in frameshift mutants with normal excision repair and did not occur in those base-pair substitution mutants tested. The results are(More)
Reaction of the rodent carcinogen acrylamide (AM) at pH 7.0 and 37 degrees C for 10 and 40 days with 2'-deoxyadenosine (dAdo), 2'-deoxycytidine (dCyd), 2'-deoxyguanosine (dGuo), and thymidine (dThd) resulted in the formation of 2-formamidoethyl and 2-carboxyethyl adducts via Michael addition. The alkylated 2'-deoxynucleoside adducts isolated (% yield after(More)
Propylene oxide (PO) is a direct-acting mutagen and rodent carcinogen. We have studied how PO modifies 2'-deoxynucleosides at pH 7.0-7.5 and 37 degrees C for 10 h. PO reacts as an SN2 alkylating agent by forming the following 2-hydroxypropyl (HP) adducts: N6-HP-dAdo (7% yield), 7-HP-Gua (37%) and 3-HP-dThd (4%). Alkylation at N-3 of dCyd resulted in(More)
The 3-carbon epoxide glycidol (GLC) was reacted with dCyd and dThd at pH 7.0 to 7.5 and 37 degrees C for 10 h. The only product detected from the reaction with dCyd was 3-(2,3-dihydroxypropyl)-dUrd (3-DHP-dUrd) whose structure was established from UV spectra, isobutane chemical ionization (CI) mass spectra together with accurate mass measurements and(More)
The effects of UV, diethyl sulfate, and 2-aminopurine in causing back mutations were studied with spontaneous and 2-aminopurine-induced leucine auxotrophs ofSalmonella typhimurium. A correlation was noted between the effectiveness of diethyl sulfate and 2-aminopurine. All the 2-aminopurine inducible mutants fell into two categories; those whose frequency of(More)
Isopropyl methanesulfonate (IPMS), an SN1 alkylating agent, is a direct-acting mutagen in bacteria. We recently reported that s.c. and topical administration of IPMS to mice resulted in the rapid induction of thymic lymphomas. Thymic lymphoma induction was not observed following administration of the SN2 alkylating agents methyl methanesulfonate (MMS) and(More)
Af-Hydroxy-1-aminonaphthalene (JV-hydroxy-1-AN), N-hydroxy-2-aminonaphthalene (N-hydroxy-2-AN), 1-aminonaphthalene (1-AN), 2-aminonaphthalene (2-AN), and 2-amino1,4-naphthoquinone were repeatedly injected i.p. into rats at a dose of 5 mg/100 gm. Lower doses of 2-amino-l-naphthol (0.15 mg/100 gm), and of #-hydroxy-aniline (1.3 mg/100 gm) were also given i.p.(More)
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