Learn More
We have used polyclonal and monoclonal antibodies raised against calf thymus profilin to localize the corresponding protein in translocating, spreading, and stationary rat fibroblasts. Immunofluorescence of whole cells and immunogold labeling on ventral membranes of lysis-squirted cells showed that profilin was markedly enriched in the highly dynamic(More)
Expression of profilin and profilin:actin ratios in vertebrates were determined with polyclonal antibodies against profilin and actin. Profilin was detected in a wide variety of bovine tissues and was enriched in smooth muscle of bovine, porcine and avian origin. The protein was purified from pig stomach muscle tissue. Smooth muscle profilin was found to be(More)
OBJECTIVES We sought to evaluate the performance of a newly designed percutaneous catheter with expandable pump. BACKGROUND The device was designed as a percutaneously insertable temporary support system for patients with acute left ventricular failure. METHODS The pump catheter (introduction diameter 9-F) is positioned in the left ventricle. The rotor(More)
Seven hundred ninety-eight patients with symptomatic single-vessel disease who underwent percutaneous transluminal coronary angioplasty (PTCA) between 1977 and 1985 were reevaluated by questionnaire 78 +/- 23 months after dilatation. Indication for PTCA was stenosis of > or = 70%, anginal symptoms, and objective signs of myocardial ischemia. The immediate(More)
AIM The development of a percutaneously insertable catheter pump for temporary left ventricular and circulatory support in patients with acute ischemic cardiomyopathy. MATERIAL AND METHODS The expandable axial flow pump (introduction diameter 9-F, expanded diameter 6.5 mm) is mounted at the tip of a catheter, designed to be placed in aortic transvalvular(More)
Phosphofructokinase and gelsolin-like proteins coexist in many muscle and non-muscle tissues. They are both actin-binding proteins, and some of their biochemical parameters are remarkably similar. In a previous report [Füchtbauer, A., Jockusch, B. M., Leberer, E. & Pette, D. (1986) Proc. Natl Acad. Sci. USA 83, 9502-9506] it was shown that(More)
The microfilament system is thought to provide motor elements needed for plasma membrane dynamics. This article focuses on two protein components that may play key roles in this process: (1) Profilin, a G-actin binding protein which is considered as the source of actin subunits necessary for rapid changes in the amount of actin filaments. Our data(More)
We have investigated the effect of caldesmon, a Ca2+/calmodulin-regulated actin-binding protein, on the complex between profilin and G-actin (profilactin). We found that smooth muscle caldesmon dissociates this complex rapidly and induces the polymerization of the released actin. Native profilactin (e.g. the complex isolated from calf thymus) proved more(More)
  • 1