Francisco Hernández

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Dissociation of protein-containing structures by modification of protein amino groups with dicarboxylic acid anhydrides is a mild procedure which, in some cases, offers advantages over treatment with alternative dissociating agents, such as urea, guanidine hydrochloride, detergents, high ionic strength, and extremes of pH: In addition to dissociating(More)
Yeast 60S ribosomal subunits have been dissociated by reversible modification with dimethylmaleic anhydride. Treatment with 40 μmol reagent/ml releases 35% of the protein, producing core particles inactive in polyphenylalanine synthesis, which are totally or highly deficient in 17 different proteins. This preparation of residual particles recovers 45% of(More)
Modification of 40S ribosomal subunits from Saccharomyces cerevisiae with dimethylmaleic anhydride (DMMA Abbreviation: DMMA, 2,3-dimethylmaleic anhydride. ), a reagent for protein amino groups, is accompanied by loss of polypeptide-synthesizing activity and by dissociation of proteins from the particles. The protein-deficient ribosomal particles, originated(More)
Modification of 60S ribosomal subunits from rat liver with dimethylmaleic anhydride (60 μol/ml) is accompanied by release of 35% of the protein. The acidic ribosomal proteins, as well as 9 basic proteins, are selectively liberated from the ribosomal subunits. Reconstitution of the protein-deficient particles with the corresponding split proteins is(More)
The contribution of lysine and arginine residues to the formation of yeast ribonucleoprotein complex 5S RNA. protein YL3 has been investigated by determining the effects on complex formation of modification with chemical reagents specific for either lysine or arginine. Treatment of protein YL3 with acetic anhydride, malefic anhydride or phenylglyoxal is(More)
Modification of calf thymus chromatin with the protein reagent dimethylmaleic anhydride is accompanied by dissociation of histones and non-histone proteins. Gel electrophoresis of the released proteins and of those bound to the residual chromatin showed that histone H1 is dissociated more easily than the core histones (H2A, H2B, H3 and H4). These are(More)
Treatment of wounds is part of the daily practice of the nursing staff and the knowledge of the physiology of healing, as well as modern treatment techniques are of great importance to provide the most effective and rapid healing method. The response of living tissues to injury constitutes the basis for understanding the process of healing. A reset of the(More)
The reversible modification of protein amino groups with dimethylmaleic anhydride, which had already been used to dissociate proteins from the 70S ribosomes of Escherichia coli (Pintor-Toro, J. A., et al. (1979) Biochemistry 18, 3219) was applied to the preparation of protein-deficient particles from the 50S subunits. Three successive cycles of treatment(More)
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