Francisco G. F. Rodrigo

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Fixation with tannic acid-glutaraldehyde permits distinction of oxytalan, elaunin and elastic fibers in the electron microscope. The results obtained using tannic acid at concentrations of 1.0%, 0.5% and 0.25% in 3% glutaraldehyde were compared. The 0.25% concentration is recommended for studying fine details of connective fibrils and for regular staining(More)
Using the tannic acid-glutaraldehyde fixation it was possible to clearly visualize, with reproducible results, the elastic fiber amorphous material because of its density. The microfibrillar component was also evidenced with a good performance. With this technique the ultrastructural patterns of the oxytalan and elaunin fibers were also demonstrated. The(More)
Epidermolysis bullosa encompasses a group of hereditary diseases clinically and pathologically characteristic. In this review the grouping criteria commonly accepted for their classification are described. The cleavage phenomena observed in these diseases are analysed in accordance with the concept of a skin area including dermal and epidermal interrelated(More)
The "elastic system" of the connective tissue includes the oxytalan, the elaunin and the elastic fibers. The oxytalan fibers are formed by the microfibrillar component of the elastic fibers (elastic microfibrils). In the human skin the oxytalan fibers are found in the papillary dermis perpendicularly oriented to the dermo-epidermal junction. It was(More)
A body of evidence suggests that glycation of proteins and the resulting fluorescent products take part in the late complications of diabetes. The purpose of this study was to analyse the fluorescence of lens soluble proteins from diabetic patients. Soluble proteins were obtained from lens with cataract from 20 type II diabetic patients and from 21 non(More)