Francis Bourgeois

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Three different mammalian myo-inositol cotransporters are currently known; two are Na+-coupled (SMIT1 and SMIT2) and one is proton-coupled (HMIT). Although their transport stoichiometries have not been directly determined, significant cooperativities in the Na+ activation of SMIT1 and SMIT2 suggest that more than one Na+ ion drives the transport of each(More)
Several different stoichiometries have been proposed for the Na(+)/monocarboxylate cotransporter SMCT1, including variable Na(+)/substrate stoichiometry. In this work, we have definitively established an invariant 2:1 cotransport stoichiometry for SMCT1. By using two independent means of assay, we first showed that SMCT1 exhibits a 2:1 stoichiometry for(More)
The accessibility of the hydrophilic loop between putative transmembrane segments XIII and XIV of the Na+/glucose cotransporter (SGLT1) was studied in Xenopus oocytes, using the substituted cysteine accessibility method (SCAM) and fluorescent labelling. Fifteen cysteine mutants between positions 565 and 664 yielded cotransport currents of similar amplitude(More)
The lipid transport system of 3-month-old male C57BL/6J obese (ob/ob) mice was investigated. Serum lipoproteins were separated by density gradient ultracentrifugation and characterized by their chemical and electrophoretic properties as well as their relative apolipoprotein contents, defined according to molecular weight and charge. Obese, ob/ob mice(More)
1. Male and female mice, 4 weeks old, were fed ad lib. diets containing various amounts of lard (0-300 g/kg) or various kinds of dietary fats (300 g/kg) for 13 weeks. Fat cell number and size were determined by a histological method in three different adipose sites. 2. Lard at 200 g/kg diet (43% energy from lipids) was sufficient to promote fat cell(More)
The effects of exposure to cholesterol in early life on diet-induced hypercholesterolemia in adult rats were investigated. Experiment 1: dams and their offspring received either a control or a cholesterol-enriched diet during gestation and lactation; at 7 weeks of age half of the rats fed each diet were switched to the other diet for 50 weeks. Experiment 2:(More)
A dynamic in vitro model was used to determine the killing kinetics of daptomycin against 15 vancomycin-resistant clinical isolates ofEnterococcus faecium. Concentration profiles simulating those observed in serum following administration of both low-dose (2 mg/kg) and high-dose (6 mg/kg) daptomycin were bactericidal within 5.5 and 2.8 h, respectively. In(More)
Effects of early over- and undernutrition on lipoprotein profiles of adult Swiss male mice reared in litters of different sizes were investigated. Lipoproteins were isolated by density gradient ultracentrifugation and defined by chemical composition. Protein moieties were defined by their changes. The lipoprotein lipase (LPL) activity in epididymal adipose(More)
195mAu, an ultrashort-lived (physical half life=30.5 s) generator-produced radionuclide, has been used in an animal model to study, by gamma camera techniques, the peripheral effects of the vasoactive drugs norepinephrine and sodium nitroprusside systemically administered or epinephrine (intraarterially) injected at various concentrations. According to the(More)
Time-kill kinetic studies were used to measure the bactericidal activity of amoxicillin/clavulanic acid (in a new form for pediatric use), cefaclor, cefuroxime, cefixime and erythromycin against 30 beta-lactamase producingMoraxella catarrhalis strains. Antibiotics were tested at the mean maximum serum concentration observed after administration of a(More)