Francesco Autuori

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Physiological deletion of cells ensues programmed death which involves formation of apoptotic bodies with fragmented DNA. Here we report that apoptotic hepatocytes are insoluble in detergents, urea, guanidine hydrochloride, reducing agents and thereby can be isolated from rat liver following collagenase treatment. They are wrinkled, spherical structures(More)
In this paper we discuss the role of "tissue" transglutaminase (tTG) in apoptosis. This enzyme by catalizing the Ca(2+)-dependent cross-linking of intracellular proteins leads to the formation of the SDS-insoluble protein scaffold in cells undergoing programmed cell death. These intracellular structures confer resistance to mechanical and chemical attack to(More)
Apoptosing cells are actively phagocytosed in parenchymal tissues, thus preventing the inflammatory reaction which could derive from their slow uncontrolled degradation. The molecular mechanisms by which an apoptotic cell is recognized and taken up are largely unknown. We propose that the recognition of apoptotic hepatocytes is mediated by the sugar(More)
We recently reported that activation of “tissue” transglutaminase (EC 2.3.2.13; tTG) in liver cells undergoing apoptosis determines extensive cross-linking of cellular proteins resulting in the formation of SDS-insoluble shells in the so-called “apoptotic bodies”. In attempt to obtain further insight into the role played by tTG in apoptosis of liver cells,(More)
The possible role of polyamines in the covalent modification of proteins in CHO cells was investigated by metabolic labeling with [3H]putrescine. A single radiolabeled protein band with an apparent relative molecular mass of 18,000 Da was observed by SDS-polyacrylamide gel electrophoresis. Almost all the radioactivity covalently linked to this protein was(More)
The authors studied the behaviour of two acid hydrolases (phosphatase and proteinase) in the liver of the larval form (ammocoetes), of the starved larva, and of the adult lamprey (Lampetra zanandreai, Vladykov). In the homogenates of liver of ammocoetes in 0.25 M sucrose the enzymatic activities are largely sedimentable (phosphatase 67%; proteinase 56%). In(More)
Computerised image analysis, performed on histological sections of (C57BL6/N) mouse lungs that had been intravenously (i.v.) injected with B16-F10 melanoma cells was used to develop a novel method to quantify the efficacy of potential antineoplastic drugs. This procedure allowed the evaluation of the rate of inhibition of growth and the anti-invasive(More)
Histochemical studies have been carried out on the composition of feltwork and tubule in the spermathecal gland cells of Dytiscus marginalis L. These extracellular structures represent the secretion-collecting apparatus of the cells of the spermathecal gland, through which the secretion passes externally into the lumen of the spermatheca. The presence of a(More)
Acid hydrolysis of trichloroacetic acid precipitate from rat tissue (liver, kidney and testis) homogenate released significant amounts of acid-insoluble putrescine, spermidine and spermine. Following incubation of liver homogenate with [1,4-14C]putrescine, 1.4% of total radioactivity and 1.0% of labelled diamine were recovered in the acid-insoluble(More)