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With the growing understanding of complex diseases, the focus of drug discovery has shifted from the well-accepted "one target, one drug" model designed towards a single target, to a new "multi-target, multidrug" model, aimed at systemically modulating multiple targets. In this context polypharmacology has emerged as a new paradigm to overcome the recent(More)
MOTIVATION The developmental stage of a cell can be determined by cellular morphology or various other observable indicators. Such classical markers could be complemented with modern surrogates, like whole-genome transcription profiles, that can encode the state of the entire organism and provide increased quantitative resolution. Recent findings suggest(More)
The cumulus cells (CCs) enveloping antral and ovulated oocytes have been regarded as putative source of non-invasive markers of the oocyte developmental competence. A number of studies have indeed observed a correlation between CCs gene expression, embryo quality, and final pregnancy outcome. Here, we isolated CCs from antral mouse oocytes of known(More)
Bioinformatics tools have been recently applied to study the differentiation of the mammalian oocyte during folliculogenesis. In this review, we will summarize our knowledge of 1) the use of biological databases for the extraction of relevant information, 2) bioinformatics methods for knowledge extraction and representation, 3) the application of these(More)
BACKGROUND Oct4 is a key factor of an expanded transcriptional network (Oct4-TN) that governs pluripotency and self-renewal in embryonic stem cells (ESCs) and in the inner cell mass from which ESCs are derived. A pending question is whether the establishment of the Oct4-TN initiates during oogenesis or after fertilisation. To this regard, recent evidence(More)
Self-renewing, chemoresistant breast cancer stem cells are believed to contribute significantly to cancer invasion, migration and patient relapse. Therefore, the identification of signaling pathways that regulate the acquisition of stem-like qualities is an important step towards understanding why patients relapse and towards development of novel(More)
Smooth muscle cells (SMCs) play critical roles in a number of diseases; however, the molecular mechanism underlying their development is unclear. Although the role of TGFβ1 signaling in SMC development is well established, the downstream molecular signals are not fully understood. We used several rat multipotent adult progenitor cell ((r)MAPC) lines that(More)