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Terpenoid Metabolism in Plastids : Isolation and Biochemical Characteristics of Capsicum annuum Chromoplasts.
- B. Camara, F. Bardat, O. Dogbo, J. Brangeon, R. Monéger
- Biology, Medicine
- Plant physiology
- 1 September 1983
A technique for the isolation and the purification of Capsicum annum L. var. Yolo Wonder chromoplasts is described. The degree of purity of the isolated chromoplasts is greatly improved by the… Expand
Sites of biosynthesis of carotenoids in Capsicum chromoplasts.
Capsicum chromoplasts incubated with isopentenyl diphosphate actively synthesize carotenoids. The enzymes involved in these reactions are compartmentalized: the stroma is the site of phytoene… Expand
Terpenoid Metabolism in Plastids Localization of α-Tocopherol Synthesis in Capsicum Chromoplasts
The synthesis of α-tocopherol from 2,3-dimethylphytylquinol and S-adenosyl-l-methionine was achieved using Capsicum annuum fruit chromoplasts. The enzymes involved in the cyclization… Expand
Metabolism of plastid terpenoids. I. Biosynthesis of phytoene in plastid stroma isolated from higher plants
Abstract Using prephytoene pyrophosphate and isopentenyl pyrophosphate as substrates, the synthesis of phytoene was demonstrated for the first time in chlorolasts, etioplasts and amyloplasts. The… Expand
Inhibition of lycopene cyclization by Capsicum chromoplast membranes by 2-aza-2,3-dihydrosqualene
Abstract 2-Aza-2,3-dihydrosqualene strongly inhibited lycopene cyclase from Capsicum chromoplast membranes.
Metabolism of plastid terpenoids: in vitro inhibition of phytoene synthesis by phenethyl pyrophosphate derivatives
The inhibition of partially purified phytoene synthetase activity from Capsicum annuum chromoplasts was investigated using aminophenethyl pyrophosphate and azidophenethyl pyrophosphate. These… Expand
Terpenoid metabolism in plastids: activity, localization and substrate specificity of chlorophyll synthetase in Capsicum annuum plastids
Rajeunissement chez le Sequoia sempervirens: effets du microgreffage in vitro
In vitro micrografting of Sequoia sempervirens was used as a rejuvenation method: apices were collected from in vitro cultured shoots issued from a 500-year-old tree; the rootstocks originated from a… Expand
Terpenoid Metabolism in Plastids : Localization of alpha-Tocopherol Synthesis in Capsicum Chromoplasts.
The synthesis of alpha-tocopherol from 2,3-dimethylphytylquinol and S-adenosyl-l-methionine was achieved using Capsicum annuum fruit chromoplasts. The enzymes involved in the cyclization… Expand
Conversion enzymatique du pyrophosphate de préphytoène en caroténoïdes par des plastes isolés
La preparation de pyrophosphate de prephytoene tritie a permis d'observer l'incorporation active de ce precurseur dans les carotenoides par les chloroplastes, les chromoplastes et les etioplastes… Expand