François Lacombe

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Confocal endomicroscopy provides tools for in vivo imaging of human cell architecture endoscopically. These technologies are a tough challenge since multiple trade-offs have to be overcome: resolution versus field of view, dynamic versus stability, contrast versus low laser power or low contrast agent doses. Many difficult clinical applications, such as(More)
A fluorescence lifetime imaging (FLIM) technology platform intended to read out changes in Förster resonance energy transfer (FRET) efficiency is presented for the study of protein interactions across the drug-discovery pipeline. FLIM provides a robust, inherently ratiometric imaging modality for drug discovery that could allow the same sensor constructs to(More)
BACKGROUND At present, immunophenotyping of hematological malignancies represents one of the most relevant clinical applications of flow cytometry. In recent years, its use has extended from clinical research to diagnostic laboratories. The aim of this report is to critically review the type of information provided by the flow cytometric immunophenotyping(More)
This article describes an original method to measure the velocity of blood cells in microvessels from a single image. The method exploits a motion artifact produced by laser scanning microscope (LSM). Although velocity can be estimated from a single image, we show how a temporal sequence can then be exploited to increase the robustness and accuracy of the(More)
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