60 | VOL.10 NO.1 | JANUARY 2013 | nature methods fluorescence microscopy using beam splitters to image up to four focal planes on separate cameras3. This method provides excellent light efficiency… (More)
Transcription is a stochastic process occurring mostly in episodic bursts. Although the local chromatin environment is known to influence the bursting behavior on long timescales, the impact of… (More)
The linker histone H1 has a fundamental role in DNA compaction. Although models for H1 binding generally involve the H1 C-terminal tail and sites S1 and S2 within the H1 globular domain, there is… (More)
Transcription factors (TFs) interact dynamically in vivo with chromatin binding sites. Here we summarize and compare the four different techniques that are currently used to measure these kinetics in… (More)
Live-cell imaging has revealed unexpected features of gene expression. Here using improved single-molecule RNA microscopy, we show that synthesis of HIV-1 RNA is achieved by groups of closely spaced… (More)
Proceedings of the National Academy of Sciences…
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Although pathways for assembly of RNA polymerase (Pol) II transcription preinitiation complexes (PICs) have been well established in vitro, relatively little is known about the dynamic behavior of… (More)
Fluorescence recovery after photobleaching (FRAP) is a widely used imaging technique for measuring the mobility of fluorescently tagged proteins in living cells. Although FRAP presumes that… (More)
Arising from E.-Z. Shen et al. 508, 128–132 (2014); doi:10.1038/nature1301210.1038/nature13012Ageing and lifespan of organisms are determined by complicated interactions between their genetics and… (More)
Mitochondrial free radicals and redox poise are central to metabolism and cell fate. Their measurement in living cells remains a major challenge and their in vivo dynamics are poorly understood.… (More)