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The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences.

• Bengt Bjellqvist, Graham J. Hughes, +5 authors Denis F. Hochstrasser
• Electrophoresis
• 1993

The focusing positions in narrow range immobilized pH gradients of 29 polypeptides of known amino acid sequence were determined under denaturing conditions. The isoelectric points of the proteins… (More)

Micropreparative two-dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins.

• Bengt Bjellqvist, Jean Charles Sánchez, +5 authors Denis F. Hochstrasser
• Electrophoresis
• 1993

We describe some simple modifications to the micropreparative two-dimensional (2-D) electrophoresis procedure using immobilized pH gradients in the first dimension and sodium dodecyl… (More)

Translationally controlled tumor protein: a protein identified in several nontumoral cells including erythrocytes.

• Jean Charles Sánchez, David E. Schaller, +7 authors Denis F. Hochstrasser
• Electrophoresis
• 1997

The translationally controlled tumor protein (TCTP) is a growth-related protein which is regulated at the translational level. It is present in mammals, higher plants and Saccharomyces cerevisiae.… (More)

Improved and simplified in-gel sample application using reswelling of dry immobilized pH gradients.

• Jean Charles Sánchez, V Rouge, +5 authors Denis F. Hochstrasser
• Electrophoresis
• 1997

A simple and inexpensive methacrylate rehydration chamber was built to accommodate ten immobilized pH gradient (IPG) strips. In the chamber, entire IPG gels were used for sample application, with the… (More)

A nonlinear wide-range immobilized pH gradient for two-dimensional electrophoresis and its definition in a relevant pH scale.

• Bengt Bjellqvist, Christian Pasquali, Florence Ravier, Jean Charles Sánchez, Denis F. Hochstrasser
• Electrophoresis
• 1993

A new nonlinear immobilized pH gradient (IPG) is proposed as the first dimension for two-dimensional electrophoresis. In comparison to conventional carrier ampholyte techniques, it offers better… (More)

Inside SWISS-2DPAGE database.

• Jean Charles Sánchez, Ron D. Appel, +4 authors Denis F. Hochstrasser
• Electrophoresis
• 1995

Several two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) databases have been established and updated for more than 15 years. Only recently have developments of computer networks and… (More)

Human liver protein map: a reference database established by microsequencing and gel comparison.

• Denis F. Hochstrasser, Severine Frutiger, +7 authors Roberto Vargas
• Electrophoresis
• 1992

This publication establishes a reference human liver protein map obtained with immobilized pH gradients. By microsequencing, 57 spots or 42 polypeptide chains were identified. By protein map… (More)

Melanie II--a third-generation software package for analysis of two-dimensional electrophoresis images: I. Features and user interface.

• Ron D. Appel, Patricia M. Palagi, +5 authors Denis F. Hochstrasser
• Electrophoresis
• 1997

Although two-dimensional electrophoresis (2-DE) computer analysis software packages have existed ever since 2-DE technology was developed, it is only now that the hardware and software technology… (More)

Plasma protein map: an update by microsequencing.

• Graham J. Hughes, Severine Frutiger, +7 authors Denis F. Hochstrasser
• Electrophoresis
• 1992

The reference plasma protein map, obtained with immobilized pH gradients in the first dimension of two-dimensional electrophoresis, is presented. By microsequencing, more than 40 polypeptide chains… (More)

Improving the detection of proteins after transfer to polyvinylidene difluoride membranes.

• Jean Charles Sánchez, Florence Ravier, +5 authors Graham J. Hughes
• Electrophoresis
• 1992

N-Terminal sequence analysis of proteins separated by two-dimensional polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride (PVDF) membranes has become the method for… (More)

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