Fernando Enríquez-Rincón

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Non-O1 strains of Vibrio cholerae implicated in gastroenteritis and diarrhea generally lack virulence determinants such as cholera toxin that are characteristic of epidemic strains; the factors that contribute to their virulence are not understood. Here we report that at least one-third of diarrhea-associated nonepidemic V. cholerae strains from Mexico(More)
This study compares the effects of passive administration of monoclonal anti-hapten (DNP) antibodies on primary plaque-forming cell (PFC) responses in mice to either soluble (DNP-keyhole limpet haemocyanin [KLH] ) or particulate (TNP-erythrocyte) antigens. IgM, IgG1, IgG2a and IgG2b antibodies at doses up to 500 micrograms induced at best a modest(More)
As part of the innate immune response NK cells destroy infected, transformed, or otherwise stressed cells within hours of activation. In contrast, CD4(+) T lymphocytes require a sustained increase in their metabolism in order to cope with the biogenesis of cell components, in a process of proliferation and differentiation into effector cells. Recently,(More)
Mice were treated from birth with either goat anti-mouse IgM or with a monoclonal anti-IgD antibody. When they were 8 weeks old, cohorts of these mice were given 125I-labelled antigen, either by itself, or in an antigen-antibody complex. Anti-IgM-treated mice, which did not develop follicular structures in their spleens, failed to retain immune complexes on(More)
The purpose of this work was to determine if anti-amebic antibody producing cell responses could be elicited in Peyer's patches and spleen in mice locally or systemically immunized with glutaraldehyde-fixed trophozoites of Entamoeba histolytica (GFT). The animals were inoculated with either a single or four doses of GFT via intragastric, rectal, and(More)
In this study we followed the in vivo fate and distribution of hapten-coupled sheep red blood cells (TNP-SRBC) coated with 125I-labelled anti-hapten antibody in mice. The majority of these complexes was rapidly taken up by the liver, and by macrophages in the marginal zone of the spleen. Within a few hours, however, marginal zone labelling diminished and(More)
A major obstacle to the study and understanding of the parasite pathogenic mechanisms and of local immunity in intestinal amoebiasis is the lack of appropriate acute models. Current in vivo models of intestinal amoebiasis produce detectable lesions only several days following the intracecal inoculation of amoebae; moreover, they are quite labor-intensive(More)
OBJECTIVE To investigate whether the HlyA-induced vacuolating effect is produced by V. cholerae O1 ElTor strains isolated from different geographic origins, including Mexico. MATERIAL AND METHODS Supernatant-induced haemolysis, vacuolating activity and cytotoxicity in Vero cells were recorded. PCR, RFLP analysis and molecular cloning were performed. (More)
The antibody responses against Entamoeba histolytica in Peyer's patches and spleen after rectal, intraperitoneal and intragastric immunization with glutaraldehyde-fixed amebas (GFA) was compared between male and female mice by the use of a spot forming cell (SFC) assay. We found that female mice elicit significant higher anti-amebic SFC responses than male(More)
We performed an immunoblot analysis of the main E. histolytica proteins recognized by immune sera and intestinal fluids of Balb/c mice immunized with glutaraldehyde fixed trophozoites (GFT) by intragastric, rectal and intraperitoneal routes, to determine if there were differences in the amebic antigens immunodominantly recognized at mucosal and systemic(More)