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sgRNA:Cas9 at the YSA gene sgRNA2 site in rice. Alleles shown were amplified from genomic DNA isolated from 6 independent T0 transgenic plants separately and sequenced after cloned into vectors. The wild type sequence is shown at the top with the PAM sequence highlighted in magenta and the target sequence in cyan. Red dashes, deleted bases; red bases,(More)
Urea represents over 50 % of nitrogen fertilizers applied worldwide to crop production, however one-third of nitrogen fertilized could be recovered by crops. Previous studies have provided useful knowledge of urea-related plant nitrogen-nutrition, whereas information about crop growth-response to urea without its external degradation seems limiting. We thus(More)
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