Fenglai Liang

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The desulfurization (dsz) genes from Rhodococcus erythropolis DS-3 were successfully integrated into the chromosomes of Bacillus subtilis ATCC 21332 and UV1 using an integration vector pDGSDN, yielding two recombinant strains, B. subtilis M29 and M28 in which the integrated dsz genes were expressed efficiently under the promoter, Pspac. The dibenzothiophene(More)
The taxonomic position of an actinomycete, strain HD321(T), isolated from an oil-producing well of Daqing oilfield, was clarified using a polyphasic taxonomic approach. The strain possessed cell-wall chemotype IV, MK-9(H(2)) as the predominant menaquinone, relatively long-chain mycolic acids (52-62 carbon atoms) of the Gordonia type, straight-chain(More)
A field experiment was performed to monitor changes in exogenous bacteria and to investigate the diversity of indigenous bacteria during a field trial of microbial enhanced oil recovery (MEOR). Two wells (26–195 and 27–221) were injected with three exogenous strains and then closed to allow for microbial growth and metabolism. After a waiting period, the(More)
Dibenzothiophene (DBT) monooxygenase (DszC) catalysis, the first and also the key step in the microbial DBT desulfurization, is the conversion of DBT to DBT sulfone (DBTO2). In this study, dszC of a DBT-desulfurizing bacterium Rhodococcus sp. DS-3 was cloned by PCR. The sequence cloned was 99% homologous to Rhodococcus erythropolis IGTS8 that was reported(More)
A thermophilic bacterial strain MS-1 was isolated from the water let from an oil well, and identified as Bacillus sp. by its 16S rDNA sequence and morphological characteristics. The bacterium was able to grow at 60 degrees C and produce extracellular polymeric substance (EPS). Analysis of the EPS showed that there was 48.3% - 54.5% polysaccharide, which was(More)
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