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In present study, a quadratic regression model based on the response surface methodology (RSM) coupled with Box-Behnken design (BBD) was employed to optimize the enzyme-assisted extraction (EAE)… Continue Reading
AIM To clone human interleukin-32(hIL-32) gene and express it in E.coli efficiently. METHODS The gene of hIL-32 was amplified by RT-PCR from human peripheral blood mononuclear cells (PBMC) which… Continue Reading
AIM To construct and express pcDNA3.1-IL-18-HSV P6 recombinant DNA vaccine, and to observe the immune responses of pcDNA3.1-IL-18-HSV P6 genetic vaccine. METHODS Genes of P6 and IL-18 were cloned… Continue Reading