Feng-lai Liang

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A thermophilic Bacillus strain NG80-2 growing within the temperature range of 45–73°C (optimum at 65°C) was isolated from a deep subterranean oil-reservoir in northern China. The strain was able to utilize crude oil and liquid paraffin as the sole carbon sources for growth, and the growth with crude oil was accompanied by the production of an unknown(More)
Dibenzothiophene (DBT) and its derivatives can be microbially desulfurized by Dsz enzymes. We investigated the expressional characteristics of the dsz operon. The result revealed that the ratio of mRNA quantity of dszA, dszB, and dszC was 11:3.3:1; however, western blot analysis indicated that the expression level of dszB is far lower than that of dszC.(More)
Dibenzothiophene (DBT) and its derivatives can be microbially desulfurized by enzymes DszC, DszA, and DszB, which are encoded by the operon dszABC and contribute to the conversion in tandem. We investigated the expression characteristics of the dsz operon. Our results revealed that the levels of transcription and translation of dszA, dszB, and dszC(More)
Strain NX02(T), a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from soil, and its taxonomic position was investigated using a polyphasic approach. Chemotaxonomic analysis revealed that strain NX02(T) possessed Q-10 as the predominant ubiquinone, sym-homospermidine as the major polyamine and C(18 : 1)omega7c, C(16 : 0) and C(14 : 0)(More)
Substituted benzothiophenes (BTs) and dibenzothiophenes (DBTs) remain in diesel oil following conventional desulfurization by hydrodesulfurization. A mixture of washed cells (13.6 g dry cell wt l−1) of Rhodococcus erythropolis DS-3 and Gordonia sp. C-6 were employed to desulfurize hydrodesulfurized diesel oil; its sulfur content was reduced from 1.26 g l−1(More)
A benzothiophene-desulfurizing strain was isolated and identified as Gordonia sp. C-6, which could degrade benzothiophene (BT) in a way analogous to the 4S pathway. It was found strain C-6 had the ability to grow in KT-BT medium whose sole source of sulfur was BT or its derivatives, whereas it couldn't grow well in a medium with DBT and its derivatives as(More)
A novel bacterium T7-2 was isolated from the oil-polluted sea-bed mud of Bohai Sea, northern China, which can degrade diesel oil at 15 degrees C. This bacterium was identified as a strain of Rhodococcus erythropolis according to its 16S rDNA gene. In order to enhance degradation efficiency, a five-level, three-factor central composite design was employed to(More)
A new bacterial strain that is capable of using diesel as the sole carbon source was isolated from the petroleum-contaminated soil of Xinjiang oil field, Northwest China. This bacterium was identified on the basis of its morphological and physiological characteristics and 16S rRNA gene sequence analysis, and it showed the greatest similarity with(More)
The amount of organic nitrides contained in fuel oil is smaller than the one of organic sulfur compounds, but the existence of them is enough to affect the invariability of oil product greatly , and has a big effect on the color of oil. They also contribute to catalyst poisoning during the refining of crude oil, thus reducing the catalyzing rate of the(More)
It is a brand-new method to isolate functional bacteria from high temperature petroleum reservoirs according to the sequence information obtained from PCR-DGGE patterns. Three-set primers of 16S rDNA high variable region, V3, V8, V9, were compared. The results showed that more microbial diversity information could be obtained from the PCR product of V9(More)