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Immunization of Pigs by DNA Prime and Recombinant Vaccinia Virus Boost To Identify and Rank African Swine Fever Virus Immunogenic and Protective Proteins
Although pigs developed clinical and pathological signs consistent with acute AsFV, viral genome levels were significantly reduced in blood and several lymph tissues in those pigs immunized with vectors expressing ASFV antigens compared with the levels in control pigs.
Antineoplastic agents. 536. New sources of naturally occurring cancer cell growth inhibitors from marine organisms, terrestrial plants, and microorganisms(1a,).
- G. Pettit, F. Hogan, +19 authors Felicia M. Craciunescu
- Biology, MedicineJournal of natural products
- 8 March 2008
The isolation of interesting and valuable cancer cell growth inhibitors including majusculamide C, axinastatin 5, bengazoles A, B, and E, manzamine A, jaspamide, and neoechinulin A has been summarized.
NMR Structure of Francisella tularensis Virulence Determinant Reveals Structural Homology to Bet v1 Allergen Proteins.
The first structure for the soluble domain of Flpp3 from the highly infectious Type A SCHU S4 strain is presented, derived through high-resolution solution nuclear magnetic resonance (NMR) spectroscopy; the first structure of a lipoprotein from the genus Francisella.
Purification and Biophysical Characterization of the CapA Membrane Protein FTT0807 from Francisella tularensis
CapA is shown to be an inner-membrane protein and is predicted to play a key cellular role in the assembly of polysaccharides.
Polyclonal Antibody Production for Membrane Proteins via Genetic Immunization
The first application of genetic immunization to a structurally diverse set of membrane proteins is described to show that immunization of mice with DNA alone produced antibodies against 71% of the bacterial and viral targets, correlated with prior reports of target immunogenicity in host organisms.
Biophysical characterization and a roadmap towards the NMR solution structure of G0S2, a key enzyme in non-alcoholic fatty liver disease
The research presented in this paper aims to aid in rational based drug design for the treatment of NAFLD by providing a pathway for a solution state NMR structure of G0S2 by describing the expression of G 0S2 in an E. coli system from two different constructs.
XFEL and NMR Structures of Francisella Lipoprotein Reveal Conformational Space of Drug Target against Tularemia.
A room temperature structure of Flpp3 determined by serial femtosecond crystallography is reported that exists in a significantly different conformation than previously described by the NMR-determined structure, paving the road to developing targeted therapeutics against tularemia.
Membrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorferi
- K. E. Robertson, C. Truong, +4 authors D. Hansen
- Chemistry, MedicineScientific Reports
- 26 November 2019
In describing the first purification of membrane-translocated BBA57, this work will support subsequent studies that reveal the precise mechanisms of this important Lyme disease virulence factor, and provide evidence for an α-helical multimer.
Biophysical Characterization of a Vaccine Candidate against HIV-1: The Transmembrane and Membrane Proximal Domains of HIV-1 gp41 as a Maltose Binding Protein Fusion
With epitopes accessible to the broadly neutralizing antibodies, these MBP/MPR-TM recombinant proteins may be in immunologically relevant conformations that mimic a pre-hairpin intermediate of HIV-1's envelope protein.
Generation of High‐Specificity Antibodies against Membrane Proteins Using DNA‐Gold Micronanoplexes for Gene Gun Immunization
- D. Hansen, Felicia M. Craciunescu, P. Fromme, S. Johnston, K. Sykes
- Chemistry, MedicineCurrent protocols in protein science
- 1 January 2018
This work describes a DNA‐based immunization method for mice that expresses the antigen in the membranes of dendritic cells, thus allowing direct presentation to the immune system and producing antibodies that recognize properly folded and stabilized forms of membrane protein antigen.