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RT-qPCR is a preferred method for rapid and reliable quantification of gene expression studies. Appropriate application of RT-qPCR in such studies requires the use of reference gene(s) as an internal control to normalize mRNA levels between different samples for an exact comparison of gene expression level. However, recent studies have shown that no single(More)
The prion hypothesis posits that a misfolded form of prion protein (PrP) is responsible for the infectivity of prion disease. Using recombinant murine PrP purified from Escherichia coli, we created a recombinant prion with the attributes of the pathogenic PrP isoform: aggregated, protease-resistant, and self-perpetuating. After intracerebral injection of(More)
Near-duplicate video retrieval is becoming more and more important with the exponential growth of the Web. Though various approaches have been proposed to address this problem, they are mainly focusing on the retrieval accuracy while infeasible to query on Web scale video database in real time. This paper proposes a novel method to address the efficiency(More)
Recently, we have provided evidence that the polymorphic self-incompatibility (S) locus-encoded F-box (SLF) protein AhSLF-S(2) plays a role in mediating a selective S-RNase destruction during the self-incompatible response in Antirrhinum hispanicum. To investigate its role further, we first transformed a transformation-competent artificial chromosome clone(More)
A class of ribonucleases termed S-RNases, which control the pistil expression of self-incompatibility, represents the only known functional products encoded by the S locus in species from the Solanaceae, Scrophulariaceae and Rosaceae. Previously, we identified a pollen-specific F-box gene, AhSLF (S locus F-box)-S 2, very similar to S 2 -RNase in(More)
A crucial component of grid-connected converters is the phase-locked loop (PLL) control subsystem that tracks the grid voltage's frequency and phase angle. Therefore, accurate fast-responding PLLs for control and protection purposes are required to provide these measurements. This paper proposes a novel feedback mechanism for single-phase PLL phase(More)
Overcurrent protection of silicon carbide (SiC) metal-oxide-semiconductor field-effect transistors (MOSFETs) remains a challenge due to lack of practical knowledge. This paper presents three overcurrent protection methods to improve the reliability and overall cost of SiC MOSFET-based converters. First, a solid-state circuit breaker (SSCB) composed(More)
Maize rough dwarf disease (MRDD) is one of the most serious virus diseases of maize worldwide, and it causes great reduction of maize production. In China, the pathogen was shown to be rice black-streaked virus (RBSDV). Currently, MRDD has spread broadly and leads to significant loss in China. However, there has been little research devoted to this disease.(More)
Staphylococcus aureus is a leading cause of food poisoning worldwide due to the production of heat-stable enterotoxins. Recently, the isolation of methicillin-resistant S. aureus (MRSA) from food animals and retail meats raised additional food safety concerns. In this study, we characterized 152 S. aureus isolates, including 22 MRSA recovered from Louisiana(More)