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A bacterial isolate, designated as DQ8, was found capable of degrading diesel, crude oil, n-alkanes and polycyclic aromatic hydrocarbons (PAHs) in petroleum. Strain DQ8 was assigned to the genus Pseudomonas aeruginosa based on biochemical and genetic data. The metabolites identified from n-docosane as substrate suggested that P. aeruginosa DQ8 could oxidize(More)
3.6. DSF-Dependent Biofilm Dispersal 167 4. DSF-Based QS Mechanisms in Other Organisms 168 4.1. Xylella fastidiosa 168 4.2. Xanthomonas axonopodis pv citri 168 4.3. Xanthomonas oryzae pv oryzae 168 4.4. Xanthomonas axonopodis pv glycines 169 4.5. Stenotrophomonas maltophilia 169 4.6. Burkholderia cepacia complex 169 4.7. Pseudomonas aeruginosa 170 5.(More)
The production of biofuels by recombinant Escherichia coli is restricted by the toxicity of the products. 2,3-Butanediol (2,3-BD), a platform and fuel bio-chemical with low toxicity to microbes, could be a promising alternative for biofuel production. However, the yield and productivity of 2,3-BD produced by recombinant E. coli strains are not sufficient(More)
Highly efficient d-lactate production by Sporolactobacillus sp. strain CASD was demonstrated in this study. Peanut meal was found to be a better nutrient than yeast extract, soybean meal, soybean peptone, corn steep, liquor beef extract, and ammonium sulfate in the production of d-lactate. To improve the utilization of peanut meal, the material was(More)
(2S,3S)-2,3-Butanediol ((2S,3S)-2,3-BD) is a crucial chiral compound that acts as an excellent building block in asymmetric synthesis of highly valuable chiral compounds. However, the low concentration and optical purity of (2S,3S)-2,3-BD produced in previous studies limited its applications. In the present work, the gene encoding 2,3-butanediol(More)
Glycerol dehydrogenase (GDH) is an important polyol dehydrogenase for glycerol metabolism in diverse microorganisms and for value-added utilization of glycerol in the industry. Two GDHs from Klebsiella pneumoniae, DhaD and GldA, were expressed in Escherichia coli, purified and characterized for substrate specificity and kinetic parameters. Both DhaD and(More)
In order to realize the full-scale sharing, free circulation and transaction, and on-demand-use of manufacturing resource and capabilities in modern enterprise systems (ES), Cloud manufacturing (CMfg) as a new service-oriented manufacturing paradigm has been proposed recently. Compared with cloud computing, the services that are managed in CMfg include not(More)
Whole cell biocatalysis is an important tool for pharmaceutical intermediates synthesis, although it is hindered by some shortcomings, such as high cost and toxicity of inducer, mass transfer resistance caused by cell membrane and side reactions. Whole-cell catalysis using N-acetyl-d-glucosamine 2-epimerase (EC 5.1.3.8) and N-acetyl-d-neuraminic acid(More)
The widely conserved second messenger cyclic diguanosine monophosphate (c-di-GMP) plays a key role in quorum-sensing (QS)-dependent production of virulence factors in Xanthomonas campestris pv. campestris. The detection of QS diffusible signal factor (DSF) by the sensor RpfC leads to the activation of response regulator RpfG, which activates virulence gene(More)
Findings from previous studies suggest that the quorum sensing signal DSF (diffusible signal factor) negatively regulates biofilm formation in Xanthomonas campestris pv. campestris (Xcc) by affecting the expression of manA encoding biofilm dispersion and an unknown factor(s). In this study, by analysing the double deletion mutant ΔrpfFΔmanA, in which DSF(More)