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We have developed a system in which a foreign antigen replaces nearly all of the surface-exposed region of the fibrillar M protein from Streptococcus pyogenes and is fused to the C-terminal attachment motif of the M molecule. The fusion protein is thus expressed on the surface of Streptococcus gordonii, a commensal organism of the oral cavity. The antigen(More)
E7 is the major transforming protein of human papillomavirus type 16 (HPV16). It has been found to associate with the retinoblastoma protein Rb1. We investigated whether HPV16 E7 protein was associated with other cellular proteins, in particular with those involved in cell cycle control. Immunoprecipitates from CaSki cell extracts with an anti E7 monoclonal(More)
Human papillomavirus type 16 E7 protein (HPV-16 E7), synthesised in Schizosaccharomyces pombe, is both phosphorylated and targeted to the nucleus [Tommasino et al., Gene 93 (1990) 265-270] as is E7 protein synthesized in primate cells. Further analysis of E7 expression in fission yeast indicates that: (i) E7 protein synthesised in S. pombe is phosphorylated(More)
The complete E7 protein-encoding open reading frame of human papillomavirus type 16 (HPV-16) was expressed in the fission yeast Schizosaccharomyces pombe, under the control of a cloned yeast promoter. The HPV-16 E7 protein synthesized in S. pombe is a 17-kDa phosphoprotein which is recognized by anti-E7 antibodies (raised in rabbits against E7 fusion(More)
Attenuating beta-amyloid precursor protein (beta-APP) gene expression may have relevance in diseases such as Alzheimer's disease, where beta-APP has been implicated in neuropathological processes. We report here on the transcriptional down-regulation of beta-APP by interferon-gamma (IFN-gamma) in SKNMC human neuroblastoma cells. Treatment of the cells with(More)
XML Schema is employed for describing the type and structure of information contained in XML documents. <i>Schema evolution</i> means that a schema is modified and the effects of the modification on instances are faced. <i>XSUpdate</i> is a language that allows to easily identify parts of an XML Schema, apply a modification primitive on them and define an(More)
Complete nucleotide sequencing of the RNAs of two unrelated neurovirulent isolates of Sabin-related poliovirus type 2 revealed that two nucleotides and one amino acid (amino acid 143 in the major capsid protein VP1) consistently departed from the sequences of the nonneurovirulent poliovirus type 2 712 and Sabin vaccine strains. This pattern of mutation(More)
N-myc and c-myc are structurally and functionally related proto-oncogenes. C-myc expression plays an essential role towards the induction of DNA replication following mitogen stimulation of quiescent cells. We have examined whether N-myc expression can stimulate cellular DNA synthesis. When growth factor-deprived quiescent Balb/c 3T3 fibroblasts harboring a(More)
Data on the Web mostly are in XML format and the need often arises to update their structure, commonly described by an XML Schema. When a schema is modified the effects of the modification on documents need to be faced. XSUpdate is a language that allows to easily identify parts of an XML Schema, apply a modification primitive on them and finally define an(More)
Induction of quiescent BALB/c 3T3 murine fibroblasts by platelet-derived growth factor (PDGF) or fibroblast growth factor (FGFs) is accompanied by induction of c-myc gene expression. To study the role of c-myc in cell growth, we transfected BALB/c 3T3 cells with a plasmid construct containing a glucocorticoid-inducible c-myc gene. When these transfected(More)