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CRISPR/Cas9-mediated DNA cleavage (CCMDC) is becoming increasingly used for efficient genome engineering. Proto-spacer adjacent motif (PAM) adjacent to target sequence is one of the key components in the design of CCMDC strategies. It has been reported that NAG sequences are the predominant non-canonical PAM for CCMDC at the human EMX locus, but it is not(More)
Clustered interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome engineering technologies are sparking a new revolution in biological research. This technology efficiently induces DNA double strand breaks at the targeted genomic sequence and results in indel mutations by the error-prone process of nonhomologous end(More)
1School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang 325027, China; 2The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou(More)
To the Editor: CRISPR-based gene editing is widely implemented in various cell types and has great potential for molecular therapy. 1 The CRISPR-Cas9 system creates sequence-specific double-strand DNA breaks that are repaired by a dominant error-prone nonhomologous end-joining (NHEJ) pathway, often resulting in gene inactiva-tion by generating frameshift(More)
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