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CRISPR/Cas9-mediated DNA cleavage (CCMDC) is becoming increasingly used for efficient genome engineering. Proto-spacer adjacent motif (PAM) adjacent to target sequence is one of the key components in the design of CCMDC strategies. It has been reported that NAG sequences are the predominant non-canonical PAM for CCMDC at the human EMX locus, but it is not(More)
To the Editor: CRISPR-based gene editing is widely implemented in various cell types and has great potential for molecular therapy. 1 The CRISPR-Cas9 system creates sequence-specific double-strand DNA breaks that are repaired by a dominant error-prone nonhomologous end-joining (NHEJ) pathway, often resulting in gene inactiva-tion by generating frameshift(More)
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