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Infections caused by community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) are being increasingly observed in patients who lack traditional risk factors. We described 8 postpartum women who developed skin and soft-tissue infections caused by MRSA at a mean time of 23 days (range, 4-73 days) after delivery. Infections included 4 cases of(More)
BACKGROUND From April to June 2001, an outbreak of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae infections was investigated in our neonatal intensive care unit. METHODS Cultures of the gastrointestinal tracts of patients, the hands of healthcare workers (HCWs), and the environment were performed to detect potential reservoirs(More)
OBJECTIVE To describe the epidemiologic and molecular investigations that successfully contained an outbreak of methicillin-resistant Staphylococcus aureus (MRSA) in a neonatal intensive care unit (NICU). DESIGN Isolates of MRSA were typed by pulsed-field gel electrophoresis (PFGE) and S. aureus protein A (spa). SETTING A level III-IV, 45-bed NICU(More)
OBJECTIVE To determine whether the molecular epidemiological characteristics of methicillin-resistant Staphylococcus aureus (MRSA) had changed in a level III neonatal intensive care unit (NICU). DESIGN Retrospective review of medical records. SETTING Level III NICU of a university-affiliated children's hospital in New York, New York. PATIENTS Case(More)
OBJECTIVE To describe the aerobic microbial flora on the hands of experienced and new graduate nurses over time. DESIGN A prospective cohort design that examined the relationship between duration of employment in an intensive care unit (ICU) and the microbial flora on the hands of experienced and new graduate nurses during a 23-month period. SETTING A(More)
OBJECTIVE When the incidence of methicillin-susceptible Staphylococcus aureus (MSSA) infection or colonization increased in our neonatal intensive care unit (NICU), we sought to further our understanding of the relationship among colonization with MSSA, endemic infection, and clonal spread. DESIGN A retrospective cohort study was used to determine risk(More)
Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae are endemic in New York City hospitals and have been associated with serious infections globally. A real-time polymerase chain reaction (RT-PCR) assay was developed to detect carbapenem resistance attributable to KPC from blood culture bottles positive for gram-negative bacilli. Culture(More)
A simple, sensitive, and specific ligation-dependent PCR (LD-PCR) method for the detection of hepatitis C virus (HCV) RNA in serum is described. The assay uses two DNA capture probes for RNA isolation and two DNA hemiprobes for subsequent PCR. Each capture probe has a 3* sequence complementary to the conserved 5* untranslated region of HCV RNA and a biotin(More)
OBJECTIVE To explore the role of the community as a potential reservoir for Acinetobacter baumannii. DESIGN Antimicrobial resistance patterns and genotypes of A. baumannii isolates from patients in two Manhattan hospitals were compared with those of A. baumannii isolates from the hands of community members. RESULTS A total of 103 isolates from two(More)
Nosocomial infections among infants in neonatal intensive care units are an increasing cause of morbidity and mortality. Efforts to rapidly detect, contain and ultimately prevent cross-transmission of these pathogens require constant vigilance by members of the epidemiology team. A vital component of an epidemiological evaluation is the ability to assess(More)