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Functional analysis of genome sequences requires methods for cloning DNA of interest. However, existing methods, such as library cloning and screening, are too demanding or inefficient for high-throughput application to the wealth of genomic data being delivered by massively parallel sequencing. Here we describe direct DNA cloning based on the discovery(More)
There are no specific treatments for patients with acute, severe neurological deficits caused by neuromyelitis optica (NMO) who fail to recover after treatment with high-dose corticosteroids. We evaluated the clinical response of anti-tuberculosis treatment (ATT) in patients suffering from steroid-refractory NMO, and investigated the correlation between NMO(More)
In this article, a novel and high efficient solvent, tetra-n-Butylammonium Hydroxide (TBAH), was used for dissolution and isolation of straw cellulose from wheat straw. The composition analysis with gas chromatography (GC) and the spectroscopic characterization analysis conducted by X-Ray diffraction (XRD)/Fourier transform infrared spectroscopy (FTIR)(More)
Business Process Execution Language for Web Services is a language, which can be used to define abstract and executable processes. It has became to be the defacto standard of Web Service composition. However, the security aspect of access control is explicitly mentioned to be outside the scope of BPEL. This paper focuses on the implementation of access(More)
Backgrounds: Tuberculoma is a granulomatous inflammatory process mimicking a neoplasm, both clinically and radiologically. Although those with an infratentorial origin are rare, this disease is still a diagnostic challenge using conventional workup. However, this disease should not be overlooked because it is essentially curable with proper diagnosis and(More)
In this paper, we focus on the frequency reuse algorithms for the downlink of fixed two-hop Orthogonal Frequency Division Multiple Access (OFDMA) cellular relaying networks which operate in decode-and-forward and half-duplex mode. An Orthogonal Resource Allocation Algorithm (ORAA) has been proposed in [1]. After analyzing the co-channel interference and(More)
Determine if different DC-SIGNR genotypes/alleles correlated with HIV susceptibility in the context of the HIV mode of infection, CD4+ T cell counts and blood HIV RNA loads. One hundred forty-five HIV infected individuals and 187 uninfected healthy controls were recruited to observe whether DC-SIGNR genotypes/alleles were correlated with HIV susceptibility,(More)
Chemokines play a paramount role in tumor progression. In hepatocellular carcinoma (HCC) progression, chemokines and their receptors play an intricate role. Currently, chemokines and their receptors such as the CXCL12-CXCR4 axis, CX3CL1-CX3CR1 axis and the CCL20-CCR6 axis have received much research attention. Although a large number of studies show that(More)
Spinosyns, the secondary metabolites produced by Saccharopolyspora spinosa, are the active ingredients in a family of insect control agents. Most of the S. spinosa genes involved in spinosyn biosynthesis are found in a contiguous c. 74-kb cluster. To increase the spinosyn production through overexpression of their biosynthetic genes, part of its gene(More)