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The wet-lab synthesis of the simplest forms of life (minimal cells) is a challenging aspect in modern synthetic biology. Quasi-cellular systems able to produce proteins directly from DNA can be obtained by encapsulating the cell-free transcription/translation system PURESYSTEM™(PS) in liposomes. It is possible to detect the intra-vesicle protein production(More)
While every attempt is made to ensure that the information in this publication is correct, no liability can be accepted by the authors or publishers for loss, damage or injury caused by any errors in, or omission from, the information given. v Preface The CoSMoS workshops series has been organised to disseminate best practice in complex systems modelling(More)
In this paper, we apply a recently developed stochastic simulation platform to investigate the dynamic behaviour of minimal 'self-(re-)producing' cellular systems. In particular, we study a set of preliminary conditions for appearance of the simplest forms of autonomy in the context of lipid vesicles (more specifically, lipid-peptide vesicles) that enclose(More)
This paper is a theoretical attempt to gain insight into the problem of how self-assembling vesicles (closed bilayer structures) could progressively turn into minimal self-producing and self-reproducing cells, i.e. into interesting candidates for (proto)biological systems. With this aim, we make use of a recently developed object-oriented platform to carry(More)
Spherical bounded structures such as those formed by surfactant aggregates (mostly micelles and vesicles), with an inside that is chemically and physically different from the outside medium, can be seen as primitive cell models. As such, they are fundamental structures for the theory of autopoiesis as originally formulated by Varela and Maturana. In(More)
In previous works we have explored the dynamics of chemically reacting proto-cellular systems, under different experimental conditions and kinetic parameters, by means of our stochastic simulation platform 'ENVIRONMENT'. In this paper we, somehow, turn the question around: accepting some broad modeling assumptions, we investigate the conditions under which(More)
In this article we present novel aspects of the impact that synthetic biology (SB) can express in a field traditionally based on computer science: information and communication technologies (ICTs), an area that we will consider taking into account also possible implications for artificial intelligence (AI) research. In the first part of this article we will(More)
Contemporary biological cells are highly sophisticated dynamic compartment systems which separate an internal volume from the external medium through a boundary, which controls, in complex ways, the exchange of matter and energy between the cell's interior and the environment. Since such compartmentalization is a fundamental principle of all forms of life,(More)