Fabiano Cruz Peixoto

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When analyzing sequencing reads, it is important to distinguish between putative correct and wrong bases. An open question is how a PHRED quality value is capable of identifying the miscalled bases and if there is a quality cutoff that allows mapping of most errors. Considering the fact that a low quality value does not necessarily indicate a miscalled(More)
In EST projects it is extremely important to be able to identify the informative region of the read, by trimming the non informative ones. Several methods for sequence trimming are available. In this work we present a methodology to compare such methods, based on the sequencing of defined sequences (pUC18 cloning vector) and homology searches to find the(More)
PHRED is the most frequently used base caller algorithm in genome projects. An interesting point on PHRED utilization is the fact that a low score on some base may not actually correspond to a miscalling on that base, but it may stand for a putative error on the region around this base. In order to evaluate the efficiency of PHRED on base calling and base(More)
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