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The biogenic amines present in the carotid body Type 1 cells of two avian species (Japanese quail and chicken) were identified, by microspectrofluorometry of formaldehyde-induced fluorescence, as dopamine and 5-hydroxytryptamine respectively. These and other cytochemical properties establish the cells as members of the APUD series. Grafts of the neural(More)
Salamanders regenerate their tails after amputation anywhere along their length. How the system faithfully reconstitutes the original number of segments and length is not yet known. To gain quantitative insight into how the system regenerates the appropriate length, we amputated tails at 4 or 16 myotomes post-cloaca and measured blastema size, cell cycle(More)
Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program(More)
Fluorescence excitation and emission spectra are reported of the fluorophores derived from various catecholamines, tryptamines and related substances treated with vapours of acetaldehyde, glutaraldehyde and acetic acid. In most cases, the excitation maxima were at about 400–410 nm, and emission maxima at about 530 nm (catecholamines) or 560 nm(More)
Kinetic characteristics of alkaline phosphatase (EC 3.1.3.1) were determined in cryostat sections of rat kidney by microfluorometry with α-naphthyl phophate as substrate, and the results were compared with measurements on enzyme extracted from this tissue. The apparent Michaelis constant of the enzyme in cryostat sections was found to be 0.6mM, in good(More)
A fluorescence metachromatic modification of the ‘masked basophilia’ method is described. It is based on the acridine dye Coriphosphine O. Excitation and emission spectra of green (orthochromatic) and red (metachromatic) fluorescent tissue components are presented. When the method is applied to suitably fixed sections, metachromasia is demonstrable in cells(More)
Die Zellen maligner Melanome, Grenznaevuszellen und normale Melanocyten zeigen in gefrier-getrockneten Gewebsschnitten eine durch Formaldehyd hervorgerufene Fluorescenz. Dieselbe Fluorescenz konnte dann nachgewiesen werden, wenn die Gewebsstücke normal Formol-fixiert und in Paraffin eingebettet waren. Mikrospektrofluorimetrische Untersuchungen zeigten, daß(More)