F. Winston

Publications181
h-index74
Citations24,222
Highly Influential Citations1,597
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Methods in Yeast Genetics: A Laboratory Course Manual
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Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex.
TLDR
The function of Gcn5 as a hist one acetyltransferase within the Ada and SAGA adaptor complexes indicates the importance of histone acetylation during steps in transcription activation mediated by interactions with transcription activators and general transcription factors.
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Construction of a set of convenient saccharomyces cerevisiae strains that are isogenic to S288C
A set of GAL2+ yeast strains that are isogenic to strain S288C have been constructed. They contain non‐reverting mutations in genes commonly used for selection for recombinant plasmids. Strains from…
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A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.
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DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs.
TLDR
The combination of biochemical studies on DSIF and genetic analysis of Spt4 and Spt5 in yeast indicates that DSIF associates with RNA Pol II and regulates its processivity in vitro and in vivo.
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Transcription Elongation Factors Repress Transcription Initiation from Cryptic Sites
TLDR
It is suggested that Spt6 plays a critical role in maintaining normal chromatin structure during transcription elongation, thereby repressing transcription initiation from cryptic promoters, and other elongation and chromatin factors appear to contribute to this control.
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Chromatin- and Transcription-Related Factors Repress Transcription from within Coding Regions throughout the Saccharomyces cerevisiae Genome
TLDR
The results show that a large number of factors that control chromatin structure and transcription are required to repress cryptic transcription from at least 1,000 locations across the S. cerevisiae genome, and two results suggest that some cryptic transcripts are translated.
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Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae.
TLDR
New genetic and biochemical studies of Saccharomyces cerevisiae Spt4, Spt5, and Spt6 reveal a role for these proteins in transcription elongation and provide strong evidence that these factors are important for transcription elongations in vivo.
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Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae.
TLDR
Chromatin immunoprecipitation assays show that H3 Lys4 methylation is present at the rDNA locus and that Set1-mediated H3 lysine 4 (Lys4)methylation is required for repression of RNA polymerase II transcription within rDNA, and suggest that Set 1-mediatedH3 Lys 4 methylation are required for normal cell growth and transcriptional silencing.
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Functional Organization of the Yeast SAGA Complex: Distinct Components Involved in Structural Integrity, Nucleosome Acetylation, and TATA-Binding Protein Interaction
TLDR
The data demonstrate that SAGA harbors multiple, distinct transcription-related functions, including direct TBP interaction and nucleosomal histone acetylation, which are highly detrimental to growth and transcription.
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