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An anticoagulant solution for haemolymph collection and prophenoloxidase studies of penaeid shrimp (Penaeus californiensis)
Abstract 1. 1. An anticoagulant solution was designed from data on osmolality, ionic concentration and pH to resemble shrimp haemolymph. 2. 2. This Shrimp Salt Solution (SSS) prevents coagulation andExpand
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Activation of the prophenoloxidase system of the brown shrimp Penaeus californiensis Holmes)
Abstract The prophenoloxidase (proPO) system of the brown shrimp Penaeus californiensis was activated using different preparations and inductors. In shrimp, this system is confined inside theExpand
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Prophenoloxidase from brown shrimp (Penaeus californiensis) hemocytes.
Prophenoloxidase (proPO) was purified from blood cells of the brown shrimp Penaeus californiensis by ultracentrifugation and dye affinity chromatography. The isolated proPO is a 114-kDa monomericExpand
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A single WAP domain-containing protein from Litopenaeus vannamei hemocytes.
A cDNA clone coding for a single WAP domain (SWD) protein was isolated from a hemocyte cDNA library of Litopenaeus vannamei. The full-length cDNA sequence is 0.4kb long and encodes a 93-amino acidExpand
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Purification and comparison of beta-1,3-glucan binding protein from white shrimp (Penaeus vannamei).
A beta-glucan binding protein (BGBP) was identified in both white (Penaeus vannamei) and blue shrimp (P. stylirostris) plasma. White shrimp BGBP was purified by affinity chromatography usingExpand
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A plasma protein isolated from brown shrimp (Penaeus californiensis) which enhances the activation of prophenoloxidase system by beta-1,3-glucan.
A beta-glucan-binding protein (BGBP) has been identified in brown shrimp plasma by using a polyclonal antiserum against a BGBP from the freshwater crayfish. The protein was purified by immunoaffinityExpand
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Effect of calcium on the prophenoloxidase system activation of the brown shrimp (Penaeus californiensis, Holmes)
Abstract The soluble prophenoloxidase (proPO) system of the brown shrimp (Panaeus californiensis) was obtained by centrifuging hemocytes (15,000 g) in low salt buffers. In these samples, proPOExpand
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Purification and characterization of the clotting protein from the white shrimp Penaeus vannamei
Abstract The protein responsible for clot formation was isolated from plasma of the white shrimp Penaeus vannamei by affinity chromatography in a heparin–agarose column. The protein, named clottingExpand
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A lipopolysaccharide-binding agglutinin isolated from brown shrimp (Penaeus californiensis Holmes) haemolymph
Abstract 1. 1. Haemolymph from the brown shrimp (Penaeus californiensis Holmes) agglutinates erythrocytes of different vertebrate species. 2. 2. A fraction containing haemagglutinating activity wasExpand
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Molecular cloning of a beta-glucan pattern-recognition lipoprotein from the white shrimp Penaeus (Litopenaeus) vannamei: correlations between the deduced amino acid sequence and the native protein
The hemolymph pattern-recognition beta-glucan binding protein from the white shrimp Penaeus (Litopenaeus) vannamei is also a high density lipoprotein (betaGBP-HDL) involved in innate immunity. TheExpand
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