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An efficient virus concentration method and RT-nested PCR for detection of rotaviruses in environmental water samples.
TLDR
The combination of the virus concentration method and RT-nested PCR described below made it possible to effectively detect rotaviruses in environmental water samples.
Detection and characterization of hepatitis A virus in water samples in Thailand
TLDR
The application of virus concentration method and reverse transcriptase‐nested polymerase chain reaction (RT-nested PCR) to detect HAV RNA and analyse the genetic sequence of the virus in environmental water samples is described.
Bacterial causes of AIDS-associated diarrhea in Thailand.
TLDR
The study found that the isolation rates of bacterial enteropathogens causing diarrhea in APD were considerably lower than those in NAPD, and the least effective antibiotics were ampicillin, tetracycline and cotrimoxazole.
Detection of Salmonella invA gene in shrimp enrichment culture by polymerase chain reaction.
TLDR
The results indicated that incorporation of an enrichment step prior to PCR has the potential to be applied for detection of naturally contaminated salmonellae in food, environment and clinical samples.
Antimicrobial Resistance, Extended-Spectrum β-Lactamase Productivity, and Class 1 Integrons in Escherichia coli from Healthy Swine.
TLDR
It is confirmed that swine can be a reservoir of ESBL-producing E. coli harboring class 1 integrons, which may become a potential health risk if these integrons are transmitted to humans and intensive analyses of animal, human, and environmental isolates are needed to control the spread.
Detection of poliovirus, hepatitis A virus and rotavirus from sewage and water samples.
TLDR
A modified adsorption-elution technique for concentration of enteric viruses from sewage and water samples was developed and poliovirus was not found by isolation in cell culture, but rotavirus antigen was found by ELISA.
Rapid detection of polioviruses in environmental water samples by one-step duplex RT-PCR.
TLDR
Poliovirus detection in environmental water collected from 2 communities in Bangkok, Thailand during February and May 1998 was more sensitive, rapid, simple and cost-effective than the cell culture technique since the two water samples which were positive for polioviruses by RT-PCR failed to be detected by cell culture.
An improved method for concentrating rotavirus from water samples.
TLDR
Reconcentration of water samples by a speedVac concentrator showed significantly higher rotavirus recovery than polyethylene glycol precipitation through both negatively and positively charged filters (p-value <0.001).
Noroviruses in oysters from local markets and oyster farms in southern Thailand.
TLDR
Findings indicate that NoV contamination in oysters obtained from both markets and oyster farms might pose a potential risk of acute gastroenteritis associated with raw oyster consumption.
Bed rails and endotracheal tube connectors as possible sources for spreading Acinetobacter baumannii in ventilator-associated pneumonia patients.
TLDR
The results demonstrate that multiple clones of MDR A. baumannii were widely spread in the hospital and bed rails and contaminated endotracheal tube connectors could be potential sources of A. Baumanni spread.
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