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Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system.
Results show the usefulness of the aequorin system in monitoring [Ca2+]i response to extracellular stimuli in yeast cells.
Aequorea green fluorescent protein
Localization, trafficking, and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells.
The GFP-human glucocorticoid receptor efficiently transactivated the mouse mammary tumor virus promoter in response to dexamethasone at 30 degrees C but not at 37 degrees C, indicating that temperature is important, even for function of the GFP fusion protein.
Chemical nature of the light emitter of the Aequorea green fluorescent protein.
  • H. Niwa, S. Inouye, F. Tsuji
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences…
  • 26 November 1996
The results indicate that the GFP Chromophore consists of an imidazolone ring structure and that the light emitter is the singlet excited state of the phenolate anion of the chromophore.
Light Production in the Luminous Fishes Photoblepharon and Anomalops from the Banda Islands
The unresolved mechanism of light production in Photoblepharon and Anomalops has been reinvestigated and it is concluded that the light is produced by symbiotic luminous bacteria and that the bacteria are of a primitive type called bacteroids.
The Luminescent Systems of Pony Fishes
The anatomy of bioluminescent organs and mode of light production in 18 species of pony fish have been investigated using fresh and preserved material and a simple classification of the luminescent systems is proposed.
Cloning and sequence analysis of cDNA for the luminescent protein aequorin.
The primary structure of the protein deduced from the nucleotide sequence showed that the protein is composed of 189 amino acid residues and has three E-F hand structures that are characteristic for Ca2+-binding sites.