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Phylogeny and Molecular Identification of Vibrios on the Basis of Multilocus Sequence Analysis
Overall, the three genetic loci were more discriminatory among species than were 16S rRNA sequences, and it can conclude that strains of the same species will have at least 98, 94, and 94% rpoA, recA, and pyrH gene sequence similarity, respectively.
Re-evaluating prokaryotic species
The current and future impact of multilocus nucleotide-sequence-based approaches to prokaryotic systematics are discussed and the potential, and difficulties, of assigning species status to biologically or ecologically meaningful sequence clusters are considered.
Inferring the Evolutionary History of Vibrios by Means of Multilocus Sequence Analysis
ABSTRACT We performed the first broad study aiming at the reconstruction of the evolutionary history of vibrios by means of multilocus sequence analysis of nine genes. Overall, 14 distinct clades…
Detection and sequencing of Zika virus from amniotic fluid of fetuses with microcephaly in Brazil: a case study.
Ecological diversification in the Bacillus cereus Group.
How adaptation to novel environments by the modification of temperature tolerance limits has shaped historical patterns of global ecological diversification in the B. cereus Group is illustrated.
Vibrio coralliilyticus sp. nov., a temperature-dependent pathogen of the coral Pocillopora damicornis.
The YB1T strain, the aetiological agent of tissue lysis of the coral Pocillopora damicornis, was characterized as a novel Vibrio species on the basis of 16S rDNA sequence, DNA-DNA hybridization data, AFLP and GTG5-PCR genomic fingerprinting patterns and phenotypic properties, including the cellular fatty acid profile.
Updating the Vibrio clades defined by multilocus sequence phylogeny: proposal of eight new clades, and the description of Vibrio tritonius sp. nov.
This study presents a better picture of an updated molecular phylogeny for 86 described vibrio species and 10 genome sequenced Vibrionaceae strains, using 8 housekeeping gene sequences.
Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes.
Both rpoA and pheS genes can be used as reliable tools for identification of clinical and environmental species of Enterococcus and are efficient screening methods for the detection of novel species.
Microbial genomic taxonomy
- C. Thompson, L. Chimetto, R. Edwards, J. Swings, E. Stackebrandt, F. Thompson
- BiologyBMC Genomics
- 23 December 2013
The application of the new genomic species definition put forward here will allow researchers to use genome sequences to define simultaneously coherent phenotypic and genomic groups.
Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism.
- F. Thompson, B. Hoste, K. Vandemeulebroecke, J. Swings
- BiologySystematic and applied microbiology
The genomic diversity among 506 strains of the family Vibrionaceae was analysed using Fluorescent Amplified Fragments Length Polymorphisms (FAFLP) indicating that many culturable taxa of the Vibrions remain as yet to be described.