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De Novo Computational Design of Retro-Aldol Enzymes
Using new algorithms that rely on hashing techniques to construct active sites for multistep reactions, retro-aldolases that use four different catalytic motifs to catalyze the breaking of a carbon-carbon bond in a nonnatural substrate are designed. Expand
Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein Kaede.
A molecular mechanism for green-to-red photoconversion, which is assisted by the water molecule is proposed, assisted by a well-defined water molecule in the proximity of the His62 imidazole ring in the green form. Expand
Catalytic antibodies as designer proteases and esterases.
  • F. Tanaka
  • Chemistry, Medicine
  • Chemical reviews
  • 18 October 2002
Enamine-based organocatalysis with proline and diamines: the development of direct catalytic asymmetric Aldol, Mannich, Michael, and Diels-alder reactions.
The contributions of this laboratory to converting enzymatic enamines, and in some cases imines, into a versatile catalytic asymmetric strategy powered by small organic molecules are summarized. Expand
Direct catalytic asymmetric synthesis of anti-1,2-amino alcohols and syn-1,2-diols through organocatalytic anti-Mannich and syn-aldol reactions.
This study compliments and extends the bioorganic approach to asymmetric synthesis in these two versatile synthon classes through direct asymmetric Mannich, Mannich-type, and aldol reactions involving unmodified α-hydroxyketones in reactions catalyzed by primary amine-containing amino acids. Expand
Direct observation of an enamine intermediate in amine catalysis.
The observation of a stable enamine intermediate in the crystal structure of an aldolase antibody 33F12 in complex with a 1,3-diketone derivative provides strong evidence that fewer residues are essential for amine catalysis within the hydrophobic environments of this catalytic antibody. Expand
Catalytic single-chain antibodies possessing β-lactamase activity selected from a phage displayed combinatorial library using a mechanism-based inhibitor
Catalytic single-chain antibodies (scFvs) possessing β-lactamase activity were selected from a phage displayed combinatorial antibody library using a penam sulfone mechanism-based inhibitor ofExpand
Rapid analysis of solvent effects on enamine formation by fluorescence: how might enzymes facilitate enamine chemistry with primary amines?
In order to determine factors that facilitate the use of primary amines in biological chemistry, solvent effects on enamine formation with glycine were studied. Solvent effects were rapidly analyzedExpand
Organocatalytic direct asymmetric aldol reactions in water.
Direct asymmetric cross-aldol reactions that can be performed in water without addition of organic solvents are developed and reveal an effective design strategy for the development of aqueous organocatalytic systems. Expand
Abstrac A lipid-coated catalytic antibody was prepared by mixing aqueous solutions of antibody and synthetic glycolipids. The lipid-coated catalytic antibody is soluble in organic solvents and showedExpand