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The stability and hydrolysis of S-adenosylmethionine; isolation of S-ribosylmethionine.
The latter phase of this work revealed conditions for splitting methionine from the molecule and for hydrolysis to adenine and a hitherto unknown fragment, S-ribosylmethionine, which is a prerequisite to microbiological, nutritional, and enzymatic research involving this substance. Expand
Bacterial uracil riboside phosphorylase.
Abstract A pyrimidine nucleosidase from E. coli has been purified. It shows specificity toward uridine and catalyzes the reversible splitting of this nucleoside by phosphorolysis.
The specificity of S-adenosylmethionine derivatives in methyl transfer reactions.
S-Adenosyl-l-homocysteine was found to be a very effective inhibitor of the systems investigated and three binding sites for S-adenosylmethionine on the methyl transfer enzymes may be postulated. Expand
The yeast Candida utilis assimilates 5′-methylthioadenosine from the culture medium and converts it into intracellular S -adenosylmethionine, and specific activity recovered was lower when methyl-C 14 -labeled material was used. Expand
Effect of some proteins on the yeast cell membrane.
Yeast cells, Candida utilis, in water suspension and in the absence of electrolytes were found to be very sensitive to several proteins of moderate size, including ribonuclease, protamine, lysozyme,Expand
S-adenosylmethionine in the vacuole of Candida utilis.
The opportunity of observing a well-defined chemical compound in the vacuole of viable cells without staining or pretreatment should yield information on the structure and function of this part of the yeast cell, especially during the growth cycle. Expand
Growth and metabolism of yeast cells after lethal X-irradiation.
The yeasts Candida utilis and Saccharomyces cerevisiae were x-irradiated and incubated for 24 to 48 hours in culture medium and produced one greatly elongated, sometimes bifurcated, bud which was much larger than the parent cell. Expand
The S-n-propyl analogue of S-adenosylmethionine.
A special strain of Saccharomyces cerevisiae responded to a supplement of S-n-propyl-L-homocysteine in the culture medium by synthesizing S-adenosyl-(S-n -propyl)L-Homocysteines, the S- n-Propyl analogue of S, which was examined for its activity as n- Propyl donor by substituting it for S- adenosylmethionine in methyltransferase systems. Expand
Properties of yeast cell ghosts obtained by ribonuclease action.
Cell ghosts were obtained from Candida utilis and Saccharomyces cerevisiae by the action of some conformational isomers of pancreatic ribonuclease which passed through the cell wall and penetratedExpand