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Neuroglobin, nitric oxide, and oxygen: functional pathways and conformational changes.
TLDR
Based on the ligand-linked conformational changes discovered by crystallography, the pathways of the reactions with O2 and NO provide a framework that may account for the involvement of Ngb in controlling the activation of a protective signaling mechanism. Expand
The O2-scavenging Flavodiiron Protein in the Human Parasite Giardia intestinalis*
TLDR
It is proposed that in G. intestinalis the primary function of FDP is to efficiently scavenge O2, allowing this microaerobic parasite to survive in the human small intestine, thus promoting its pathogenicity. Expand
Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics.
TLDR
It is concluded that p (50) ranges from 0.01 to 0.10 kPa in mitochondria and small cells in the absence of inhibitors of cytochrome c oxidase, whereas experimental artefacts explain the controversial >200-fold range of p ( 50) in the literature on mitochondrial oxygen kinetics. Expand
Mitochondrial bioenergetic adaptations of breast cancer cells to aglycemia and hypoxia
TLDR
The data demonstrate that cancer cells behave differently than normal cells when adapting their bioenergetics to microenvironmental conditions, and the differences in hypoxia and aglycemia tolerance between breast cancer cells and non-cancer cells may be important when optimizing strategies for the treatment of breast cancer. Expand
Proton uptake upon anaerobic reduction of the Paracoccus denitrificans cytochrome c oxidase: a kinetic investigation of the K354M and D124N mutants.
TLDR
The kinetics and stoichiometry of the redox-linked protonation of the soluble Paracoccus denitrificans cytochrome c oxidase were investigated by multiwavelength stopped-flow spectroscopy and indicated that complete reduction of heme a (and Cu(A)) is coupled to the uptake of approximately 0.8 H(+), which is independent of both H-pathways. Expand
Endogenous Myoglobin in Breast Cancer Is Hypoxia-inducible by Alternative Transcription and Functions to Impair Mitochondrial Activity
TLDR
It is reported that MB mRNA and protein levels are robustly induced by prolonged hypoxia in breast cancer cell lines, in part via HIF-1/2-dependent transactivation, and the mitochondrion-impairing role of MB in hypoxic cancer cells is part of a novel tumor-suppressive function. Expand
Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin
TLDR
Evidence is provided that FlRD‐reductase rapidly shuttles electrons between NADH and FlRd, a prerequisite for NO reduction in this detoxification pathway, and the electron accepting site in FlRt, the Rd center, is in very fast redox equilibrium with the flavin mononucleotide. Expand
Probing the access of protons to the K pathway in the Paracoccus denitrificans cytochrome c oxidase
TLDR
It is concluded that protons do not enter the K’channel via E78II in the Paracoccus enzyme. Expand
Kinetic characterization of the Escherichia coli nitric oxide reductase flavorubredoxin.
TLDR
The amperometric and time-resolved spectroscopic approaches are presented, giving access to the study of in vitro reactivity of a complex multi-redox center enzyme. Expand
Targeted metabolomics for bioprocessing
Background Bioprocesses like the cell-based production of biologicals, i.e. mainly recombinant proteins and monoclonal antibodies, require optimal culture conditions to obtain a high yield of qualityExpand
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