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DNA sequencing with chain-terminating inhibitors.
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. Expand
Sequence and organization of the human mitochondrial genome
The complete sequence of the 16,569-base pair human mitochondrial genome is presented and shows extreme economy in that the genes have none or only a few noncoding bases between them, and in many cases the termination codons are not coded in the DNA but are created post-transcriptionally by polyadenylation of the mRNAs. Expand
Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome.
The bovine 12 S and 16 S Ribosomal RNA genes, when compared with those from human mitochondrial DNA, show conserved features that are consistent with proposed secondary structure models for the ribosomal RNAs. Expand
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
A simple and rapid method for determining nucleotide sequences in single-stranded DNA by primed synthesis with DNA polymerase is described and was used to determine two sequences in bacteriophage φX174 DNA. Expand
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
An approach to DNA sequencing using chain-terminating inhibitors (Sanger et al., 1977) combined with cloning of small fragments of DNA in a single-stranded DNA bacteriophage is described, determining the 2771-nucleotide sequence of the largest MboI restriction enzyme fragment from human mitochondrial DNA. Expand
Nucleotide sequence of bacteriophage phi X174 DNA.
A DNA sequence for the genome of bacteriophage phi X174 of approximately 5,375 nucleotides has been determined using the rapid and simple 'plus and minus' method. The sequence identifies many of theExpand
Nucleotide sequence of bacteriophage φX174 DNA
The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs. Expand
Nucleotide sequence of bacteriophage lambda DNA.
The distribution of other rare codons in the genes of the left arm suggests that they may have a controlling function on the relative amounts of the proteins produced, and the genome is fairly compact with comparatively little non-coding DNA. Expand
The nucleotide sequence of bacteriophage φX174
The complete nucleotide sequence of the DNA of bacteriophage φX174 has been determined and the amino acid sequences of the ten proteins for which the DNA codes have also been deduced. Expand